"Time-nested acclimation to excitation pressure: the transcriptome, proteome and phosphoproteome"

地球上几乎所有生物的最终能量来源是阳光。植物和藻类的叶绿体在将所有其他生物体与太阳联系起来方面至关重要，它们能够吸收、捕获和转化光能，将二氧化碳还原为复杂的碳水化合物。这需要将极快的、对温度不敏感的光化学反应与消耗这种能量的慢得多的、依赖于温度的代谢过程相结合，以捕获能量。因此，光合生物倾向于表现出细胞能量收支的不平衡，这可以在体内量化为激发压力（EP）。细胞能量收支问题的精致解决方案反映了光合作用的部分魔力，并且是植物和藻类动态能力的结果，以重塑光合装置的结构和功能以适应不断变化的环境，以便通过最小化EP来保持能量平衡。我的研究计划的长期目标是阐明的分子基础，植物和藻类的感觉，并响应于EP的短期变化，由于环境光和温度条件的改变，并整合这些信息随着时间的推移，以重塑光合装置，并产生在长期，稳态驯化和适应EP的表型变化。拟议的研究将确定叶绿体内存在的EP的潜在分子传感器的性质和数量，以及这些传感器不仅改变光合机构的结构，而且改变模式植物拟南芥的表型的机制。这将与研究相结合，利用独特的光合装置的一种新的，冷适应，非模型，南极绿色鱼，衣原体raudensis UWO 241，阐明其调节光合电子流调制EP的动态机制。 拟议的研究将改变我们的传统观点的结构和功能的光合机构，不仅作为一个动态的全球细胞能量传感器，但也作为一个动态的能量Transformer。

The ultimate source of the energy for almost all organisms on this planet is sunlight. The chloroplasts of plants and algae are crucial in linking all other living organisms to the sun through the ability to absorb, trap, and transform this light energy to reduce CO2 to complex carbohydrates. This requires the integration of extremely fast, temperature-insensitive photochemical reactions to trap the energy with much slower, temperature-dependent metabolic processes that consume this energy. As a consequence, photosynthetic organisms are predisposed to exhibit an imbalance in cellular energy budget which can be quantified in vivo as excitation pressure (EP). Exquisite solutions to the problem of cellular energy budget reflect part of the magic of photosynthesis and are the result of the dynamic capacity of plants and algae to remodel the structure and function of the photosynthetic apparatus to an ever-changing environment in order to maintain an energy balance by minimizing EP. The long-term objective of my research programme is to elucidate the molecular basis by which plants and algae sense and respond to short-term changes in EP due to alterations in environmental light and temperature conditions and integrate this information over time to remodel the photosynthetic apparatus and generate a phenotypic change during long-term, steady-state acclimation and adaptation to EP. The proposed research will identify the nature and number of potential molecular sensors of EP present within chloroplasts and the mechanism(s) by which these sensors alter not only the structure of the photosynthetic apparatus but also the phenotype of the model plant, Arabidopsis thaliana. This will be combined with research that exploits the unique photosynthetic apparatus of a novel, cold-adapted, non-model, Antarctic green alga, Chlamydomonas raudensis UWO241, to elucidate the dynamic mechanism by which it regulates photosynthetic electron flow to modulate EP. The proposed research will alter our conventional view of the structure and function of the photosynthetic apparatus, not only as a dynamic global cellular energy sensor but also as a dynamic energy transformer.