Microbial community response to biostimulation in a petroleum hydrocarbon contaminated site in Saskatoon, SK

我们建议描述并开发一个概念模型，以描述SK Saskatoon碳氢化合物污染含水层与生物刺激相关的微生物群落变化。该含水层在几十年的时间里被一个加油站污染，是该项目行业合作伙伴联邦合作社有限公司（FCL）管理的许多类似地点之一。这些网站对FCL的成员合作社来说是一个长期的负担，他们渴望改进他们用来修复这些网站的方法。为了实现这一目标，重要的是要全面了解当地地质、污染物分布、含水层地下水和土壤的微生物学以及随着时间的推移生物刺激对系统的影响之间的相互作用。我们将使用高通量扩增子测序来表征6个月生物刺激期地下水中微生物群落的变化。我们还将描述从现场采集的岩心样本，以检查生物刺激之前土壤中的原位微生物学和矿物学。这些数据，连同FCL提供的地球化学分析，将用于构建由生物刺激驱动的含水层生物地球化学变化的概念模型。这将有助于我们了解微生物如何对该位点的生物刺激做出反应，并改进FCL用于修复该位点和类似位点的策略。

We propose to characterize and develop a conceptual model for microbial community changes associated with biostimulation of a hydrocarbon contaminated aquifer in Saskatoon, SK. The aquifer was contaminated by a fuel station over a period of several decades, and is one of many similar sites managed by the industry partner on this project, Federated Cooperatives Limited (FCL). These sites present a long-term liability for FCL's member co-operatives, and they are keen to refine the approaches they use to remediate these sites. To achieve this objective, it is important to have a holistic understanding of the interplay between the local geology, the distribution of the contaminants, the microbiology of the aquifer groundwater and soils, and the impact of biostimulation amendments on the system through time. We will use high-throughput amplicon sequencing to characterize microbial community changes in the groundwater at the site over a 6 month biostimulation period. We will also characterize core samples taken from the site to examine the in-situ microbiology and mineralogy in the soils prior to biostimulation.These data, along with geochemical analyses provided by FCL, will be used to construct a conceptual model for the biogeochemical changes driven by biostimulation in the aquifer. This will help us to build our understanding of how microbes respond to biostimulation at this site and and improve the strategies FCL uses for remediation of this and similar sites moving forward.