Light Scattering for Nanoscale Analysis of Molecular Machines and Extracellular Vesicles
Light Scattering for Nanoscale Analysis of Molecular Machines and Extracellular Vesicles
批准号:
RTI-2016-00515
负责人:
Gibbings, Derrick
金额:
$10.91万
依托单位:
依托单位国家:
加拿大
项目类别:
Research Tools and Instruments
财政年份:
2015
资助国家:
加拿大
项目状态:
已结题
起止时间:
2015-01-01 至 2016-12-31
中文摘要
光散射(LS)能够分析从0.3 nm(~1 kDa)到10 μm(1 MDa)的物体的绝对质量、大小和形状的动态变化,包括细胞外囊泡、蛋白质和多分子复合物。该技术可用于微量比色皿中的溶液或与任何色谱柱串联,以研究洗脱复合物。这使得可以高度准确地分析这些对象之间的相互作用及其对分子环境(pH值,辅因子,化学物质)变化的敏感性。这种对物体动态变化的纳米级分辨率使各种各样的变革性应用成为可能。
英文摘要
Light scattering (LS) enables analysis of dynamic changes in the absolute mass, size and shape of objects from 0.3 nm (~1 kDa) to 10 μm (1 MDa) including extracellular vesicles, proteins and multimolecular complexes. This technology can be used either with solutions in a microcuvette or in-line with any column to investigate eluting complexes. Together this allows highly accurate analysis of the interactions between these objects and their sensitivity to changes in their molecular environment (pH, co-factors, chemical). This nanoscale resolution of dynamic changes in objects enables a remarkable variety of transformative applications.
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会议论文
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批准号:436104-2013
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依托单位:
国内基金
海外基金
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