Microscopy suite for time-lapse multi-wavelength live cell imaging

用于延时多波长活细胞成像的显微镜套件

• 批准号: 473101-2015
• 负责人: Gibbings, Derrick
• 金额: $ 10.93万
• 依托单位: University of Ottawa
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2014
• 资助国家: 加拿大
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=564915
• 关键词: Microscopy; suite; time; lapse; multi

This application is for funding the purchase of a suite of instruments to microinject molecules into living cells and image the ensuing processes in a temperature controlled environment. There is currently no dedicated imaging platform equipped with an automated micro-injection system in Roger Guindon Hall. The lack of such infrastructure considerably limits the progress of several laboratories interested in addressing basic questions regarding subcellular organization and trafficking, such as nucleolar architecture, endosome and autophagosome trafficking, mitochondrial organization and other cellular processes. Given the transient or short-lived nature of these processes and architectures, the absence of a live-cell imaging system equipped with an automated micro-injector severely limits the ability of researchers in Roger Guindon Hall to utilize many innovative tools such as advanced fluorescent sensors of cellular processes, peptides, and interfering antibodies for example. This equipment is a feature of all modern biology research departments and will allow many researchers at the University of Ottawa to progress towards making fundamental discoveries in the life sciences. While helping several research laboratories in the Faculty, this platform will also expand the repertoire of techniques mastered by the numerous post-doctoral fellows, graduate and undergraduate students supervised in our laboratories.

这项申请是为了资助购买一套仪器，将分子显微注射到活细胞中，并在温度控制的环境中成像随后的过程。罗杰·金登大厅目前没有配备自动微量注射系统的专用成像平台。缺乏这种基础设施大大限制了几个实验室在解决有关亚细胞组织和贩运的基本问题方面的进展，这些基本问题包括核仁结构、内体和自噬小体贩运、线粒体组织和其他细胞过程。鉴于这些过程和体系结构的暂时性或短暂性，缺乏配备自动微量注射器的活细胞成像系统严重限制了罗杰·金登大厅的研究人员利用许多创新工具的能力，例如先进的细胞过程、多肽和干扰抗体的荧光传感器。这种设备是所有现代生物学研究部门的特色，将使渥太华大学的许多研究人员在生命科学的基础发现方面取得进展。在帮助学院的几个研究实验室的同时，这个平台还将扩大我们实验室监督的众多博士后研究员、研究生和本科生掌握的技术储备。