Developing High Speed Scanning Electrochemical Microscopy of Biological Substrates

JANINE MAUZEROLL（麦吉尔UNIVERSITY; PIN：290829） 提案摘要 在我们的研究中，我们使用电化学来研究化学和生物系统。我们有兴趣开发基于电化学的成像方法，可用作诊断工具。我们的方法是多方面的，因为它结合了生物学，分析化学，材料科学和显微镜的大量专业知识。 在即将到来的赠款期间，我们将利用我们在过去6年中在3个相互关联的系统上取得的进展。 (I)我们将开发一种高速电分析方法，以量化和映射与发生在具有异质电化学活性的表面（腐蚀合金，电池）的关键氧化还原过程相关的材料通量。这种尖端的电化学成像技术作为能够跟踪关键癌症相关转运泵活性的诊断测定是有希望的。 (II)我们将设计基于氧化还原活性磷脂的智能药物递送纳米材料。我们的目标是探索这类新的磷脂的电化学和自组装。 (III)我们将致力于开发分析电化学成像策略，以研究模式化癌细胞的多药耐药性。我们的目标是开发一种定量的多药耐药性检测方法，能够评估II中开发的纳米材料中封装的抗癌药物的作用。

JANINE MAUZEROLL (MCGILL UNIVERSITY; PIN: 290829) Summary of Proposal In our research we use electrochemistry to study chemical and biological systems. We are interested in developing electrochemical based imaging methods that can be used as diagnostic tools. Our approach is multifaceted as it combines considerable expertise in biology, analytical chemistry, material sciences, and microscopy. In the coming grant period, we will capitalize on our progress over the past 6 years working on 3 interrelated systems. (I) We will develop a high-speed electroanalytical methodology to quantify and map material fluxes related to key redox processes occurring at surfaces with heterogeneous electrochemical activity (corroding alloys, cells). Such cutting-edge electrochemical imaging techniques are promising as diagnostic assays capable of tracking the activity of key cancer-related transporter pumps. (II) We will design smart drug delivery nanomaterials based on redox active phospholipids. Our goal is to explore the electrochemistry and self-assembly of this new class of phospholipids. (III) We will pursue development of analytical electrochemical imaging strategies to study multidrug resistance of patterned cancer cells. Our goal is to develop a quantitative multidrug resistance assay capable of evaluating the effects of anticancer agents encapsulated in the nanomaterials developed in II.