Multi-User Ultra High Performance Liquid Chromatography/Mass Spectrometry Instrument for High-Throughput Analysis

许多科学家需要分析和识别化学品和生物化学品，以支持具有广泛社会和环境目标的研究计划。随着新的、在某些情况下结构复杂的化学和生化材料的设计、制备和分离，需要更有能力的技术来鉴定它们。为了实现这一目标，不断发展的液相色谱和质谱学领域通过最先进的设备提供了改进的分析模式。 为了满足实际研究界对能够轻松监测化学反应和生化方案的进展和成功的能力的需求，需要为连接到能够裂解的质谱仪(MS/MS)的超高效液相色谱(UHPLC)系统提供资金。 所要求的仪器将为来自多个小组的研究人员提供第一个高通量手段，用于监测反应和方案，从而快速分析结果。所要求的仪器将取代目前正在使用的费力的工作台方法(TLC)，从而使研究人员能够对他们正在接受培训的行业中常规使用的技术积累经验。 所要求的多用户UHPLC/MS/MS仪器将设在Dalhousie化学质谱仪(DCMSF)。这个设施已经运营了13年，艾莉森·汤普森博士是这里的共同申请者，担任主任。该设施由一名全职达尔豪西专家工作人员提供支持，并提供专门的专门实验室空间和一间办公室。通过DCMSF提供的强大、长期和成功的操作模式将被用作管理和维护所要求的UHPLC/MS/MS的手段；在这样做的情况下，该仪器将保持在研究就绪状态，供需要为其研究项目实际操作的高吞吐量和详细数据的受训者使用。 申请是代表使用该设施的研究社区提交的。在过去的四年里，该设施已经满足了5个省15个机构的40个研究小组的需求：将仔细管理所请求的UHPLC/MS/MS，以确保最大限度地使用、使用效率和最佳的研究洞察力。 所要求的UHPLC/MS/MS将使研究受训人员能够学习如何使用现代分析设备来进一步实现以下领域的研究目标： ·为绿色技术发明改进的催化剂； ·探索新的结构多样性，将其作为药物发现的工具； ·表征蛋白质和氨基酸等生物分子的分子行为； ·确定用于早期疾病检测的生物标志物； ·描述化学反应的机制；以及 ·合成新的功能材料。

Many scientists need to analyze and identify chemicals and biochemicals to support research programs with broad-ranging societal and environmental goals. As new and in some cases structurally complex chemical and biochemical materials are designed, prepared and isolated, more capable techniques are required for their identification. Towards this goal, the ever-evolving fields of liquid chromatography and mass spectrometry provide improved modes of analysis, as made available via state-of-the art equipment. Funds are requested for an ultra high performance liquid chromatography (UHPLC) system coupled to a mass spectrometer (MS) capable of fragmentation (MS/MS) to satisfy a hands-on research community in need of the ability to easily monitor the progress and success of chemical reactions and biochemical protocols. The requested instrumentation will provide researchers from multiple groups with the first high-throughput means by which to monitor reactions and protocols, and thus rapidly analyze outcomes. The requested instrumentation will replace laborious bench methods (TLC) currently in use, and will thus enable researchers to become experienced with techniques that are routinely used in the industries for which they are being trained. The requested multi-user uHPLC/MS/MS instrument will be located in the Dalhousie Chemistry Mass Spectrometry Facility (DCMSF). This facility has been operating for >13 years with Dr. Alison Thompson, a co-applicant herein, as the Director. The facility is supported by a dedicated full-time Dalhousie expert staff technician, as well as through the provision of dedicated specialized laboratory space and an office. The robust, long-term, and successful operating model made available through the DCMSF will be utilized as a means of managing and maintaining the requested uHPLC/MS/MS; in doing so the instrument will be maintained in a research-ready state for trainees needing hands-on high throughput and detailed data for their research projects. The application is submitted on behalf of the research community that uses the facility. Over the past four years the facility has served the needs of >40 research groups at 15 institutions in 5 provinces: the requested uHPLC/MS/MS will be carefully managed to ensure maximum usage, efficiency of use, and optimal research insight. The requested uHPLC/MS/MS will enable research trainees to learn how to use modern analytical equipment to further research goals in areas such as: • inventing improved catalysts for green technology; • exploring novel structural diversity as a tool for drug discovery; • characterizing the molecular behaviour of biomolecules such as proteins and amino acids; • identifying biomarkers for early disease detection; • characterizing mechanisms of chemical reactions; and • synthesizing new functional materials.