UPLC System for Muscle Health Research

超高效液相色谱（UPLC）是一种允许研究人员以高灵敏度分离，鉴定和定量生物样品组分的方法。它依赖于使用压力使生物样品溶液通过柱。该溶液可以与该柱中存在的材料相互作用，并有效地将分子彼此分离，以帮助识别它们。在约克大学，我们有大量的研究人员在生物系，运动学和健康科学学院，肌肉健康研究中心（MHRC），需要使用UPLC技术。在这份申请中，我们要求资金仅用于在约克校区购买第二套此类设备系统。我们希望利用它来分析血清、肌肉和心脏等生物样本中的氨基酸、儿茶酚胺、代谢物和肽，主要是为了支持MHRC成员以及其他部门同事的研究。我的5个共同申请人和我将大量使用这台设备，作为MHRC的主任，我也代表上述3个研究单位的许多（>30）其他教职员工提交这份申请，他们进行细胞生物化学研究，并可能使用这台设备。这一设备将提高我们对复杂生物样品进行基本生化分析的能力，购置这一设备将大大有助于消除目前前往另一座大楼使用约克大学校园内另一台功能仪器的用户的僵局。

Ultra High-performance Liquid Chromatography (UPLC) is a method which allows the researcher to separate, identify and quantify the components of a biological sample with great sensitivity. It relies on the use of pressure to pass the biological sample solution through a column. This solution can interact with materials present in this column and effectively separate molecules from each other, to help identify them. At York University we have a large number of researchers in the Department of Biology, the School of Kinesiology and Health Science, and the Muscle Health Research Centre (MHRC) that require the use of UPLC technology. In this application we are requesting funds to acquire only the second such equipment system on the York campus. We wish to use this to analyze amino acids, catecholamines, metabolites and peptides in biological samples such a serum, muscle, and heart primarily to support the research of MHRC members, but also our colleagues in other departments. My 5 co-applicants and I will make heavy use of this equipment, and as the Director of the MHRC, I am submitting this application also on behalf of the numerous (>30) other faculty members in the 3 research units above who do cellular biochemistry research and could potentially make use of this equipment. This equipment will increase our capacity for the fundamental biochemical analyses of complex biological samples, and its acquisition markedly help to dissipate the logjam of users who currently travel to another building to use the one other functional instrument on the York University campus.