Physiological constraints on DNA replication and fidelity

生命的蓝图编码在有机体的DNA中；这个遗传程序决定了为了维持有机体的生长和繁殖将产生什么分子。细菌基因组的复制、修复和(在某些情况下)损伤取决于细胞中特定蛋白质的活性。值得注意的是，这些蛋白质的丰度取决于有机体生长的速度。因此，我们预计基因组稳定性和细胞生长之间存在一个迄今未被探索的内在和复杂的耦合。我们的目标是确定成长计划的成功如何影响将该计划传递给后代的保真度。

The blueprint of life is encoded in the DNA of an organism; this genetic program dictates what molecules will be produced in order to sustain growth and reproduction of the organism. The replication, repair, and (in some cases) damage of the bacterial genome is dependent upon the activity of specific proteins in the cell. What is remarkable is that the abundance of these proteins changes depending upon how rapidly the organism grows. Consequently, we expect a heretofore unexplored intrinsic and complex coupling between genome stability and cell growth. Our objective is to determine how the success of the growth program affects the fidelity of transmitting that program to subsequent generations.