Antibody Glycosylation by Mass Spectrometry

抗体(Abs)是免疫系统中最重要的糖蛋白，免疫球蛋白G(Ig G)是最常见的代表。免疫球蛋白有许多来源和几个动机来表征它们的糖基化模式和氨基酸(AA)序列，这是这一提议的中心主题。PI的实验室专门从事糖基化分析已有20多年的经验，培养了许多HQP。这项建议涉及在5年内至少10个HQP，每个HQP都将受益于生物分析化学和质谱学(MS)的跨学科培训。提案的各节摘要如下。

Antibodies (Abs) are glycoproteins of prime importance in the immune system, with immunoglobulin G (IgG) as their most common representative. There are many sources of IgGs and several motives to characterize their glycosylation patterns and amino acid (AA) sequences, which is the central theme of this proposal. The PI’s laboratory has specialized in glycosylation analysis for over 20 years, and has trained many HQP. This proposal involves a minimum of 10 HQP over 5 years and each of them will benefit of interdisciplinary training in bioanalytical chemistry and mass spectrometry (MS). Sections of the proposal are summarized below.