Characterization and selection of advanced potato varieties with multiple resistance genes against late blight

魁北克西门子公司是一家位于保护区的优质马铃薯种子生产商。因此，由马铃薯晚疫病菌引起的土豆枯萎病是一个持续的威胁，他们必须控制住才能保持他们的认证。马铃薯晚疫病通常是通过密集的杀菌剂喷洒制度来控制的，这会带来严重的健康和环境问题，而不是保护区内的马铃薯种子种植者和/或有机生产者的选择。抗性品种的使用代表了最环保的替代方案之一，但加拿大商业上可获得的马铃薯品种中没有一种对晚疫病具有足够的抗性水平。该项目旨在鉴定来自魁北克公司的先进马铃薯品种，以鉴定那些含有多个抗晚疫病基因的品种。这将通过使用与已知抗病基因相关联的分子标记，以及最先进的效应组学方法来实现，该方法包括直接测试给定品种从病原体中识别特定无毒基因的能力。最后一种方法可能导致识别新的或未知的抗源，并将有助于识别分子标记不存在的抗病基因。确定要发布的抗病品种将有助于该公司维持保护区的植物检疫条件，并使他们能够选择抗病品种进行繁殖和田间试验的最后阶段。

Semences Élites du Québec Inc. is a producer of elite seed potatoes located in a protected area. As such, potatolate blight, caused by Phytophthora infestans, represent a constant threat that they must keep under control inorder to keep their certification. Potato late blight is usually controlled through an intense fungicide sprayregime, which raises serious health and environmental issues, and is not option for seed potato growers inprotected areas and/or organic producers. The use of resistant varieties represents one of the mostenvironmentally-friendly alternative, still none of the potato varieties commercially available in Canada haveadequate levels of resistance against late blight. This project aims at characterizing advanced potato varietiesfrom Semences Élites du Québec Inc. to identify those harboring multiple genes of resistance against lateblight. This will be achieved through the use of molecular markers linked with known resistance genes,together with a state-of-the-art effectoromics approach, which consist of directly testing the ability of a givenvariety to recognize a particular avirulence gene from the pathogen. This last approach could result in theidentification of novel or unknown sources of resistance, and will be helpful to identify resistance genes forwhich molecular markers do not exist. Identifying resistant varieties for release will help the company maintainphytosanitary conditions in protected areas, as well as allowing them to select resistant varieties forpropagation and final stages of field testing.