Endogenous Fluorescence Imaging of Clinically Important Bacteria on Surfaces

建议的项目是建立体内细菌负荷的荧光成像的可行性，并研究存在的细菌的量化的可能性。主要的研究挑战是(1)确定具有在现实环境中评估细菌负荷的潜力的最佳激发，以及(2)以非侵入性的方式证明荧光成像系统在细菌负荷可视化中的有效性。为此，内源性荧光成像有能力评估临床相关细菌的细菌负荷。我们的团队在微生物表面和利用荧光光谱研究这些系统方面拥有丰富的专业知识。这一合作将帮助Oxilight开发与其多光谱成像设备相辅相成的荧光成像设备。

The proposed project is to establish feasibility of fluorescence imaging of bacterial load in vivo and toinvestigate the possibility of quantification of bacteria present. The main research challenges are (1) theidentification of the optimal excitation with potential for assessment of bacterial load in realistic settings, and(2) demonstrate the usefulness of the fluorescence imaging system for bacterial load visualization in anon-invasive manner. To this end endogenous fluorescence imaging has the capacity to assess bacterial load forclinically-relevant bacteria. Our group has significant expertise in the area of microbial surfaces and theutilization of fluorescence spectroscopy to study those systems. This collaboration would help Oxilightdeveloping fluorescence imaging modality complementary to its multispectral imaging device.