Characterization of Antigen-Adjuvant interactions by Atomic Force Microscopy

赛诺菲巴斯德正在开发新的疫苗产品，这种产品含有吸附在不同类型佐剂上的抗原。抗原-佐剂相互作用的表征和综合分析对这一发展具有重要意义。然而，传统的表征方法不允许在分子水平上测量试剂之间的分子作用力，这是关键。赛诺菲巴斯德和滑铁卢大学Zoya Leonenko博士的实验室之间拟议的新合作的目标是通过先进的生物物理方法，如原子力显微镜、原子力光谱和表面等离子体共振，来表征佐剂与抗原的相互作用，这将增强对此类相互作用所涉及的作用力的了解，并将有助于了解赛诺菲巴斯德疫苗产品中抗原吸附强度与佐剂之间的相关性。这一新知识将导致新产品开发，并将通过提供先进的疫苗来加快药物研究，从而有助于改善加拿大和世界各地的健康状况。

Sanofi Pasteur is developing novel vaccine products, which contain antigens adsorbed to different types ofadjuvants. Characterization and comprehensive analysis of the antigen-adjuvant interactions is of greatimportance for this development. Traditional ways of characterization, however do not allow measurement ofmolecular forces acting between reagents on molecular level which is of key importance.The proposed new collaboration between Sanofi Pasteur and Dr.Zoya Leonenko's laboratory at the Universityof Waterloo has a goal to characterize adjuvant-antigen interactions by advanced biophysical methods, such asAtomic force microscopy, atomic force spectroscopy, and surface plasmon resonance, which would enhancethe knowledge of the forces involved in such interactions and would help in the understanding of the relevanceof the strength of antigen adsorption to adjuvants in Sanofi Pasteur's vaccine products. This new knowledgewill lead to new product development and will help to accelerate pharmaceutical research by providing anadvanced vaccine thus will contribute to the improvement of health of Canadian and worldwide community.