Typing and Targeting Mechanisms of RNA Decay Caused by Drug Treatment

多伦多的一家名为RNA诊断的公司已经开发出一种测试，可以确定化疗是否有效。这项测试是基于肿瘤样本中RNA破坏的水平及其计算分析。RNA是从DNA复制而来的，增加的RNA有助于导致癌症中的肿瘤生长。RNA衰退是一个信号，表明化疗有效，肿瘤细胞正在死亡。然而，RNA诊断人员想要了解导致RNA衰退的途径，以及不同水平的RNA干扰是否意味着不同的药物效果或结果。多伦多Ryerson大学的Sarah Sabatinos将使用酵母细胞来测试药物治疗后RNA破坏是如何发生的。Sabatinos实验室将使用显微镜来了解RNA破坏是如何发生的，并测试是否存在其他化疗有效性的标志。我们的联合目标是使RNA诊断的工具能够更好地预测肿瘤细胞死亡，并在给药后更早发挥作用。这项工作对促进加拿大的培训和改进诊断和预测计算工具非常重要。

A Toronto-based company, Rna Diagnostics, has developed a test that determines whether chemotherapy isworking. The test is based upon the level of RNA disruption in a tumour sample, and its computationalanalysis. RNA is copied from DNA, and increased RNA can help to cause tumour growth in cancer. RNAdecay is a signal that chemotherapy is working and that tumour cells are dying. However, Rna Diagnosticswould like to understand the pathways that cause RNA decay, and whether different levels of RNA disruptionmean different drug effects or outcome.Dr. Sarah Sabatinos (Ryerson University, Toronto) will use yeast cellsto test how RNA disruption happens after drug treatment. The Sabatinos lab will use microscopy to understandhow RNA disruption happens, and test whether additional marks of chemotherapy effectiveness are present.Our combined goal is to make Rna Diagnostics' tool better able to predict tumour cell death, and work earlierafter drug is given. This work is important to promote training and improve diagnostics and predictivecomputational tools in Canada.