Value-added applications for industrial waste eggshell membrane for cosmetic application

大量的蛋壳废料是鸡蛋加工工业的副产品。处理这些蛋壳会造成环境和经济负担，因此，这些材料的替代用途将会有明显的好处。蛋壳废料可能是提取新型生物活性肽的重要增值资源，用于人类健康、化妆品、生物医学和营养保健等领域。提取有价值的蛋壳衍生成分的关键步骤是实现膜与富钙壳的清洁分离。虽然到目前为止的研究已经证明蛋壳膜衍生蛋白可能具有生物活性/营养保健潜力，但对蛋壳膜衍生肽，包括其生产和潜在的生物活性知之甚少。目的：1)探索适于工业化生产的蛋壳膜水解方法。2)研究蛋壳膜废弃物中新型肽的提取、生产和表征；3)检查它们在化妆品中的使用/应用。生物活性膜衍生多肽的生产将通过使用市售蛋白酶（包括胶原酶和alcalase）进行酶水解，以生产胶原衍生多肽/生物活性多肽。不同的酶和酶的组合将被检查，以确定肽生产的最佳方法。蛋壳膜肽在化妆品应用中的潜在活性也将通过培养的皮肤细胞系（角质形成细胞和成纤维细胞）进行检测。我们将在体外评估蛋壳膜衍生肽在胶原凝胶上的成纤维细胞增殖情况。使用体外细胞培养模型分析成纤维细胞的迁移和酶活性（氢化酶、酪氨酸酶、弹性酶等）。加拿大的蛋产业为经济创造了超过10亿美元的收入，但它很容易受到消费者对蛋的态度和观念转变的影响。因此，对加拿大蛋业来说，重要的是使鸡蛋的用途多样化，超越传统的食品用途，并开发新的增值生物制品和保健产品。

A significant amount of eggshell waste is produced as a by-product of the egg processing industry. Disposal ofthese eggshells creates an environmental and financial burden and, therefore, alternative uses for thesematerials would be of obvious benefit. Eggshell waste may be an important value-added resource for theextraction of novel bioactive peptides for human health, cosmetic, biomedical and nutraceutical applications. Acritical step to the extraction of valuable eggshell-derived components is to achieve a clean separation of themembranes from the calcium-rich shell. While studies up until now have demonstrated that eggshellmembrane-derived proteins may have bioactive/nutraceutical potential, little is known about eggshellmembrane-derived peptides, including their production and potential bioactivity. The objectives: 1) To exploremethods of eggshell membrane hydrolyzation capable of industrial production. 2) To studyextraction/production and characterization of novel peptides from eggshell membrane waste and;3) To examinetheir use/applications in cosmetic products. Production of bioactive membrane-derived peptides will then becarried out by enzymatic hydrolysis using commercially available proteases, including collagenase andalcalase, to produce collagen-derived peptides/bioactive peptides. Different enzymes and enzyme combinationswill be examined to determine the best method of peptide production. The potential activity of eggshellmembrane peptides in cosmetic applications will also be examined using cultured skin cell lines (keratinocytesand fibroblasts). The proliferation of fibroblasts on the collagen gel by eggshell membrane derived peptideswill be assessed in vitro. Fibroblasts migration and enzyme activity (hyuaronase, tyrocinase, elastase etc) willbe analyzed using in vitro cell culture model. The Canadian egg industry generates over $1 billion into theeconomy, but it is vulnerable to shifts in consumer attitudes and perceptions about eggs. It is thereforeimportant for the Canadian egg industry to diversify egg utilizations beyond traditional food uses and developnew value-added bio-products and heath products.