Combined effects of environmental factors on algal and cyanobacterial physiology

农药造成的污染是许多水生生态系统面临的实际问题。在这些水域中发现的浮游植物(藻类和蓝藻)是食物网中的第一批生物，不幸的是，它们可能对水质变化非常敏感。湖泊和河流很少受到一种污染物的影响，这些化学品的毒性可能会受到其他环境因素的影响，如光线、水温和其他物质。过去很少有研究了解这些环境应激源对藻类和蓝藻生理的联合影响。在本研究计划中，我们将研究一些农药(草甘膦、美索曲松、阿特拉津、氯硫苯胺和戊烷)组合或对其他环境因素(温度或光照)对浮游植物生理的影响。我们将集中于这些环境因素对光系统II-光系统I电子传输和替代电子途径(例如PSI循环电子流)的影响。我们还将研究浮游植物对环境因素(如非光化学能量耗散过程和抗氧化酶系统)组合的抗性机制。我们还将使用转录转录方法来补充功能数据，并进一步了解潜在的机制。*这项研究将使我们能够通过将基因表达与生理过程联系起来，更好地了解不同环境应激源对藻类和蓝藻新陈代谢的相互作用。从长远来看，这项研究可能有助于建立基于污染物与自然发生的环境因素相互作用的新的水质标准。

Pollution caused by pesticides is an actual problem for many aquatic ecosystems. Phytoplankton (algae and cyanobacteria) found in these waters are the first organisms of the food web and may, unfortunately, be very sensitive to water quality variations. Lakes and rivers are impacted rarely by only one pollutant and the toxicity of these chemicals may be affected by other environmental factors, such as light, water temperature and other substances. Very few studies were performed in the past to understand the combined effect of these environmental stressors on the physiology of algae and cyanobacteria.******* In this research program we will study the effects of some pesticides (glyphosate, mesotrion, atrazine, clothianidine and captane) when combined together or to other environmental factors (temperature or light) on phytoplankton physiology. We will focus on the impacts that these environmental factors have on photosystem II – photosystem I electron transport and alternative electron pathways (e.g. PSI cyclic electron flow). We will also study the mechanisms involved in the resistance of phytoplankton species to the combination of environmental factors (e.g. the non-photochemical energy dissipation processes and the antioxidant enzyme system). We will also use transcriptomic approach to complement the functional data and to obtain further insights in underlying mechanisms.******* This research will permit, by linking gene expression to physiological processes, to obtain a better understanding of the interaction of different environmental stressors on algal and cyanobacterial metabolism. On a long term, this research may help to establish new water quality criteria based on the interaction of pollutants with natural occurring environmental factors.******