UBC Okanagan Peptide Detection System

我的NSERC研究项目的长期目标是了解人类新陈代谢和炎症之间的相互作用。利用综合生理学方法，利用运动和营养干预来改变代谢，并研究对激素、细胞因子、代谢物和免疫细胞功能的影响，我们推进了对代谢和炎症如何整合的机制的理解。我的短期目标是：***1)确定运动强度和模式如何影响抗炎细胞因子功能，以及相关机制***2)确定营养酮症如何影响免疫细胞表型和功能***对激素和细胞因子的具体、有效和可靠的评估显然对我的研究至关重要，因为它们在每项研究中都被测量，通常作为主要结果。我们机构的其他几位nserc资助的研究人员也需要这些能力，包括合作者A. Klegeris博士（研究神经胶质相互作用的免疫学方面）和Sanjoy Ghosh博士（研究饮食脂肪酸对炎症和代谢的影响）。他们的工作需要从各种样品类型（例如，细胞培养上清液，细胞/组织裂解液和小鼠血浆）中进行高灵敏度的肽检测，通常体积有限。需要Meso Scale Discovery (MSD) Meso Quickplex SQ20平台和UBC欧肯那根肽检测系统的建立，为我们的研究项目提供最尖端、高灵敏度和特异性的技术来检测生物样品中的肽（激素和细胞因子）。这种新的研究仪器将使我们的研究团队能够以比现有系统高3到30倍的灵敏度测量循环细胞因子和激素。这是必要的，因为许多细胞因子（从细胞释放的小肽，作为炎症传播者的功能）在血液中的浓度非常低。为了了解运动和营养操作如何影响这些细胞因子，需要一种高灵敏度的检测系统来测量它们。来自多个实验室的20多名高素质人员将定期使用该设备，获得宝贵的生化分析经验，并将其应用于人体生理学，代谢和免疫学。利用这种新的研究仪器增强测量循环细胞因子和激素的能力，将对人类代谢和炎症之间的基本整合产生新的见解。

The long-term objectives of my NSERC research program are to understand the interaction between metabolism and inflammation in humans. Using an integrative physiology approach where exercise and nutritional interventions are used to alter metabolism and study the resultant effects on hormones, cytokines, metabolites and immune cell function, we advance understanding of the mechanisms underlying how metabolism and inflammation are integrated. The short-term objectives of my accompanying NSERC Discovery Grant renewal are:***1) To determine how exercise intensity and pattern impact anti-inflammatory cytokine function, and the mechanisms involved ***2) To determine how nutritional ketosis impacts immune cell phenotype and function ***Specific, valid, and reliable assessment of hormones and cytokines are clearly essential for my research as they are measured in every study, typically as the major outcomes. Several other NSERC-funded researchers at our institution also need these capabilities, including collaborators Dr. A. Klegeris (who studies immunological aspects of neuron-glial interactions) and Dr. Sanjoy Ghosh (who researches the impact of dietary fatty acids on inflammation and metabolism). Their work requires high-sensitivity peptide detection from various sample types often with limited volumes (e.g., cell culture supernatants, cell/tissue lysates, and mouse plasma). The Meso Scale Discovery (MSD) MESO Quickplex SQ20 platform and the establishment of the UBC Okanagan Peptide Detection System is needed to provide our research programs with the most cutting-edge, highly sensitive, and specific technology for detecting peptides (hormones and cytokines) in biological samples.***This new research instrument will enable our research teams to measure circulating cytokines and hormones at an increased sensitivity of ~3 to 30-fold higher than current systems. This is essential because many cytokines (small peptides that are released from cells and function as inflammatory communicators) are present in very low concentrations in the blood. In order to understand how exercise and nutritional manipulations influence these cytokines a high-sensitivity assay detection system is needed to be able to measure them. Over 20 highly qualified personnel from several labs will use the equipment regularly gaining valuable biochemical assay experience with application to human physiology, metabolism, and immunology. Enhancing the ability to measure circulating cytokines and hormones with this new research instrument will yield new insights into the fundamental integration between metabolism and inflammation in humans.