Development of Technologies for Rapid Isolation and Genome Sequencing of Single Circulating Tumor Cells in Prostate Cancer

绝大多数癌症死亡是由转移引起的，或者说是癌症从身体的一个部位扩散到另一个部位。为了使癌症扩散，被称为循环肿瘤细胞的细胞必须从肿瘤中分离出来，在血流中循环，并重新附着到不同的组织上。因此，循环肿瘤细胞被认为是转移因子，** 可能用于评估每个患者癌症的遗传基础以及它们 ** 可能对潜在治疗的反应。循环肿瘤细胞的遗传分析目前受到这些细胞在血流中的极端稀有性以及个体细胞之间的差异的限制。该项目旨在通过开发一种技术 ** 来解决这一挑战，以快速分离单个循环肿瘤细胞进行基因组测序。然后，我们将通过分离和测序来自目前参加相关临床研究的 ** 晚期前列腺癌患者的单个循环肿瘤细胞来测试 ** 这项技术。然后将来自单个循环肿瘤细胞的基因组 ** 序列与其他可用数据进行比较，以确定其在诊断和治疗中的价值。基因组测序已经被用于某些癌症，以预测患者对某些药物的反应。该项目将 ** 开发一种技术来分离和测序与转移最相关的细胞，** 这将为医生提供更好的信息来评估每个患者的疾病，** 以便制定个性化的治疗计划。

The vast majority of cancer deaths are caused by metastasis, or the spread of cancer from**one part of the body to another. In order for cancer to spread, cells known as circulating tumor**cells, must detach from a tumor, circulate in the bloodstream, and reattach to a different**tissue. Therefore, circulating tumor cells are considered to be the agents of metastasis that**could potentially be used to evaluate the genetic basis of each patients cancer and how they**might respond to potential treatments. Genetic analysis of circulating tumor cells is currently**limited by the extreme rarity of these cells in the bloodstream, as well as by the differences**across individual cells. This project aims to address this challenge by developing a technology**to rapidly isolate individual circulating tumor cells for genome sequencing. We will then test**this technology by isolating and sequencing single circulating tumor cells from patients with**advanced prostate cancer who are currently enrolled in related clinical studies. The genome**sequence from single circulating tumor cells will then be compared with other available data in**order to determine its value in diagnosis and treatment. Genome sequencing is already being**used in some cancers to predict how patients will respond to certain drugs. This project will**develop a technology to isolate and sequence cells that are most relevant to metastasis,**which will give physicians significantly better information to evaluate each patients disease in**order to devise a personalized treatment plan.