Improving the reliability of cytology diagnosis with immunoplasmonic multiplex labeling

大多数癌症诊断首先是通过活检确定的，因此，活检对加拿大人的医疗保健至关重要。不同类型的活检是为了达到诊断，但医生更喜欢细针穿刺细胞学，以下简称为 ** 细胞学，因为它与患者的副作用较少。因此，肺和胰腺等许多器官每年进行的细胞学检查数量正在增加。细胞学由病理学家评估，他们使用不同的工具来完善他们的诊断。最常用的工具的目的是检测细胞中对癌症特异的蛋白质。必须执行多个制备步骤来使这些蛋白质着色，这可能会破坏所述蛋白质，从而影响工具的可靠性。因此，我们的目标是通过使用金属纳米颗粒来绕过制备步骤，以在不降解蛋白质的情况下识别蛋白质，从而提高诊断的可靠性。此外，我们建议使用特定的基于激光的技术来检测金属 ** 纳米颗粒。该项目可以被认为是开创性的，因为对人类 ** 细胞学样本的临床验证将确保诊断的高度有益准确性和 ** 治疗的最佳选择，从而改善加拿大患者的健康。为了实现这一创新的研究项目，我们组建了一个优秀的多学科和互补的团队，由国际知名的科学家和工业合作伙伴组成，他们来自该项目所涉及的全方位知识。该团队已经在 ** 金属纳米颗粒的使用和生物诊断检测方面获得了强大的专业知识，我们被 ** 同行公认为蛋白质检测专家。我们的卓越表现体现在多项专利 ** 和发明声明上。我们的工业合作伙伴，光子等，完善了我们在金属纳米粒子及其检测的商业化方面的技能。

Most cancer diagnoses are first established using a biopsy, and as such, biopsies are critical**to the health care of Canadians. Different types of biopsies are performed to achieve a**diagnosis, but physicians do prefer fine-needle aspiration cytology, hereafter referred to as**cytology, because it is associated with fewer side effects for the patients. Accordingly, the**number of cytology performed annually is increasing for many organs such as lung and**pancreas. Cytology is evaluated by pathologists, who are using different tools to refine their**diagnoses. The objective of the most commonly used tool is to detect proteins in the cells**which are specific for cancer. The multiple preparation steps that have to be performed to**colour those proteins can destroy said proteins and therefore affect the reliability of the tool.**Our objective is therefore to bypass the preparation steps by using metallic nanoparticles to**identify the proteins without degrading them, thus improving the reliability of the diagnosis.**Furthermore, we propose to use specific laser-based technologies to detect the metallic**nanoparticles. This project may be considered pioneering as clinical validation on human**cytology samples will ensure highly beneficial accuracy of diagnosis and optimal selection of**treatment, consequently improving the health of Canadian patients. To achieve this innovative**research project, we put together an excellent multidisciplinary and complementary team of**internationally renowned scientists and industrial partners coming from the full spectrum of**knowledge involved in this project. This team has already acquired a strong expertise in**metallic nanoparticles use and detection for biological diagnostics and we are recognized by**our peers as protein detection experts. Our excellence is demonstrated by multiple patents**and declarations of inventions. Our industrial partner, Photon etc, completes our skills in**commercialization of metallic nanoparticles and their detection.