Value-added applications of eggshell membrane-derived bioactive peptides for cosmetic application

加拿大每年生产约90亿个鸡蛋，其中约27%是在破蛋时加工的，副产品是低价值的蛋壳。加拿大家禽业每年产生约1.5吨蛋壳膜（ESM）废物。发现新的蛋壳废物增值应用将具有重要的经济和环境效益。由于对牛海绵状脑病（BSE）的关注，蛋壳源性胶原蛋白和胶原源性肽是牛胶原蛋白的有吸引力的替代品。只有少数应用被报道，包括吸附重金属离子的基质，烧伤的生物敷料，细胞培养的底物和酶固定化的平台。需要进一步的研究来验证ESM肽在特定化妆品应用中的生物活性。假设：esm肽可用于化妆品，包括皮肤移植，刺激人体皮肤成纤维细胞的生长，滋润，美白，减少皮肤炎症的产生。目的：1)利用ESM肽评价成纤维细胞在胶原凝胶上的增殖能力；2)在体外细胞培养模型中评价成纤维细胞的迁移能力和酶活性（氢化酶、酪氨酸酶、弹性酶等）。该项目的主要目标是1)促进成纤维细胞增殖，2)抗炎，3)美白。成纤维细胞迁移是维持技能弹性和水分的重要因素，我们测量了ESM肽对细胞增殖和氢氧化酶/酯酶活性的影响。抗炎活性将通过检测TNF-？MCP-1和IL-6是人类技能中典型的炎症介质，它们与皮肤抗衰老有关。最后，我们将通过检测酪氨酸酶活性和抗紫外线作用来检测ESM肽的美白活性。该项目的最终目标是从工业废料中开发出有价值的ESM肽。ESM衍生的生物材料对世界市场有吸引力，个人对猪或牛来源的材料有宗教上的关注。

Canadian produces about 9 billion eggs per year and about 27% are processed at egg breaking which yield lowvalue eggshell as a by-product. The Canadian poultry industry produces about 1.5 tons eggshell membrane(ESM) waste annually. The discovery of novel value-added applications for eggshell waste would haveimportant financial and environmental benefits. Eggshell-derived collagen and collagen-derived peptides are anattractive replacement for bovine collagen due to the concern over bovine spongiform encephalopathy (BSE).Only a few applications have been reported, including matrices for adsorption of heavy metal ions, biologicaldressings for burns, substrates for cell culture and platforms for enzyme immobilization. Further studies arerequired to validate the biological activity of ESM peptides for specific cosmetic application. Hypothesis: ESMpeptides can be applied in cosmetics including skin grafts, stimulate the growth of human skin fibroblasts,moisture, whitening and reduce the production of inflammation in skin. The objectives: 1)To assess theproliferation of fibroblasts on the collagen gel by ESM peptides in vitro, 2) To evaluate fibroblasts migrationand enzyme activity (hyuaronase, tyrocinase, elastase etc) in vitro cell culture model. The main targets of thisproject are 1) enhance fibroblast proliferation, 2) anti-inflammation and 3) whitening. Fibroblast migration isimportant factor for maintaining skill elasticity, moisture and we measure the effect of ESM peptides on thecell proliferation and hyuaronase/estase activity. Anti-inflammatory activity will be measured by testing TNF-?,MCP-1, IL-6 which are typical inflammatory mediators in human skill and they are related to anti-aging ofskin. Lastly, we will measure whitening activity of ESM peptides by means of testing tyrocinase activity andanti-UV effect. The ultimate goal of the project is to develop valued added ESM peptides from industrial wasteESM. ESM derived biomaterials are attractive to world markets where individuals have religious concernsregarding porcine or bovine sourced material.