Combined effects of environmental factors on algal and cyanobacterial physiology

农药污染是许多水生生态系统面临的现实问题。在这些水域中发现的浮游植物（藻类和蓝藻）是食物网的第一批生物，不幸的是，它们可能对水质变化非常敏感。湖泊和河流很少只受到一种污染物的影响，这些化学物质的毒性可能受到其他环境因素的影响，例如光、水温和其他物质。在过去，很少有研究是为了了解这些环境压力因素对藻类和蓝藻的生理的综合影响。*******在这个研究项目中，我们将研究一些农药（草甘膦、中效磷、阿特拉津、噻虫胺和captane）在一起使用或与其他环境因素（温度或光照）一起使用时对浮游植物生理的影响。我们将重点关注这些环境因素对光系统II光系统I电子传递和替代电子途径（例如PSI循环电子流）的影响。我们还将研究浮游植物对环境因素（如非光化学能量耗散过程和抗氧化酶系统）组合的抗性机制。我们还将使用转录组学方法来补充功能数据，并进一步了解潜在的机制。*******这项研究将允许，通过将基因表达与生理过程联系起来，以更好地了解不同环境应激源对藻类和蓝藻代谢的相互作用。从长远来看，这项研究可能有助于根据污染物与自然环境因素的相互作用建立新的水质标准。******

Pollution caused by pesticides is an actual problem for many aquatic ecosystems. Phytoplankton (algae and cyanobacteria) found in these waters are the first organisms of the food web and may, unfortunately, be very sensitive to water quality variations. Lakes and rivers are impacted rarely by only one pollutant and the toxicity of these chemicals may be affected by other environmental factors, such as light, water temperature and other substances. Very few studies were performed in the past to understand the combined effect of these environmental stressors on the physiology of algae and cyanobacteria.******* In this research program we will study the effects of some pesticides (glyphosate, mesotrion, atrazine, clothianidine and captane) when combined together or to other environmental factors (temperature or light) on phytoplankton physiology. We will focus on the impacts that these environmental factors have on photosystem II photosystem I electron transport and alternative electron pathways (e.g. PSI cyclic electron flow). We will also study the mechanisms involved in the resistance of phytoplankton species to the combination of environmental factors (e.g. the non-photochemical energy dissipation processes and the antioxidant enzyme system). We will also use transcriptomic approach to complement the functional data and to obtain further insights in underlying mechanisms.******* This research will permit, by linking gene expression to physiological processes, to obtain a better understanding of the interaction of different environmental stressors on algal and cyanobacterial metabolism. On a long term, this research may help to establish new water quality criteria based on the interaction of pollutants with natural occurring environmental factors.******