Multimode Microplate Reader with Imaging Capabilities

我们体内有不同的细胞类型，它们在全身功能中扮演着非常不同的角色。无论是脑细胞、免疫细胞、肌肉细胞还是循环胎儿细胞，了解它们在不同环境中的行为以及它们如何与身体其他部位沟通，将拓宽我们对正常功能的理解，以及对异常功能的可能治疗策略。我们集体研究计划的中心焦点是确定由几种不同的生理模型引起的蛋白质含量，活性和细胞位置的变化。为了更好地研究每个细胞单独或与另一个细胞结合，重要的是要有必要的设备，使这些测量在细胞或亚细胞水平。在某些情况下，这些测量不仅需要了解细胞内或细胞之间发生了什么，而且需要了解它发生在哪里。我们希望在我们各自的研究领域解决这些问题，使用一种新的仪器，Cytation 5MV细胞成像多模式酶标仪，这将使我们能够在各种细胞和组织类型中进行与RNA和蛋白质含量，蛋白质功能和抗体存在相关的核心测量。它是市场上最先进的此类仪器之一，因为它将自动数字成像与传统的多模式微孔板阅读相结合，以提供细胞信息和定量数据。它还将使我们能够看到这些事件在正常情况下以及在不同的细胞环境下发生的位置。新的仪器还将使我们能够检查任何环境影响（例如，运动、饮食和药物）改变细胞功能。** 每年，我们在实验室培训2030名高素质人员（HQP）。使用细胞成像酶标仪是他们培训的基础，因为他们将学习只有使用该系统才能学习的新技术。该仪器的功能将丰富我们的研究成果，允许在同一个样本中的多种措施，并允许更多的访问设备的HQP。Cytation 5MV细胞成像多模式酶标仪对于我们的集体研究计划和及时完成HQP项目至关重要。**我们的研究项目采取综合的，自上而下的方法，鼓励HQP欣赏在生物体，系统，组织，细胞和分子水平的生理过程的调节。总之，我们的研究有助于了解代谢疾病，神经变性，炎症，骨骼肌健康和性取向的生物学机制。

There are different cell types in our body that play very different roles as they relate to whole-body function. Whether it be brain, immune, muscle, or circulating fetal cells, knowing how they behave in different environments and how they communicate with the rest of the body will broaden our understanding of normal function and possible treatment strategies for abnormal function. The central focus of our collective research programs is determining changes in protein content, activity, and cellular location induced by several different physiological models. To better study each cell in isolation or in conjunction with another cell, it is important to have the necessary equipment to make these measurements at cellular or subcellular levels. In some cases, these measurements require an understanding not only of what is happening within a cell or between cells but where it is happening.******We wish to address these questions in our respective research areas with a new instrument, the Cytation 5MV Cell Imaging MultiMode Microplate Reader, that will allow us to make core measures associated with RNA and protein content, protein function, and antibody presence in a variety of cell and tissue types. It is one of the most advanced instruments of this kind on the market as it combines automated digital imaging with conventional multimode microplate reading to provide cellular information and quantitative data. It will also allow us to see where these events are taking place under normal circumstances and when presented with differing cellular environments. The new instrumentation will also allow us to examine any environmental influences (e.g., exercise, diet, and pharmaceuticals) that alter cell function. ******Each year, we train 2030 highly qualified personnel (HQP) in our labs. Access to the cell-imaging microplate reader is foundational to their training in that they will learn new techniques that are only possible with this system. The features of the instrument will enrich our research findings by allowing for a multitude of measures within the same sample, and allow for greater access to equipment by HQP. The Cytation 5MV Cell Imaging MultiMode Microplate Reader is essential for our collective research programs and for the timely completion of HQP projects.******Our research programs take an integrative, top-down approach that encourages HQP to appreciate the regulation of physiological processes at the organism, systems, tissue, cell and molecular levels. Together, our research contributes novel approaches toward understanding the biological mechanisms underpinning metabolic diseases, neurodegeneration, inflammation, skeletal muscle health, and sexual orientation.**