NMR Systems Upgrade for a Sustainable New Era

麦吉尔大学化学系有8台核磁共振仪器，每年支持该系32个不同研究小组的300多名化学HQP的培训和研究。此外，还有来自McGill和加拿大其他学术机构的其他部门的许多其他HQP和PI，其研究需要使用这些核磁共振仪器。因此，本NSERC RTI的五位共同申请者所要求的氦回收仪器是代表麦吉尔大学化学系核磁共振仪器的所有用户提交的。所要求的仪器是核磁共振研究可持续新时代的必要升级，并将允许最佳利用PIS的NSERC发现补助金，以最大限度地提高研究生产率和HQP培训。*五个共同申请者的研究应用实例包括：一般使用核磁共振表征小分子、表征与小配体的生物分子相互作用以及为了解催化转化而进行的机理研究。Arndtsen的研究重点是开发新的过渡金属催化反应，范围从金属催化、成键化学、多组分耦合反应到C-H键功能化。因此，他的HQP完全依赖于使用核磁共振分析来监控反应和催化剂的开发。格里森的研究重点是用于一系列合成转化的新型有机催化剂，如多烯环化、氧合和环加成化学。他的研究包括探索基本的潜在机制，这需要仔细的动力学分析，包括广泛使用高场核磁共振的13C同位素实验。李的研究重点是非传统和新颖的化学转化，可以将化学合成的效率改变为更绿色和可持续的化学。他的每一个研究项目都涉及广泛的液体和固体核磁共振，化合物的表征，以及为机械研究人员使用核磁共振。Tsantrizos的研究旨在开发影响酶和化学介导的催化过程的分子识别元件。她的团队正在设计一种含磷的分子，这种分子可以用作酶抑制剂，也可以作为金属介导和有机催化不对称合成的配体。在这些研究过程中，需要通过核磁共振研究来阐明配体的酶结合构象和催化剂-底物之间的相互作用。Tempe的研究重点是阐明涉及PINK1和Parkin的蛋白质-蛋白质相互作用，这两种蛋白质在与帕金森氏症有关的线粒体质量控制途径中协同工作。他的研究需要广泛的核磁共振HSQC滴定实验，使用15N和13C标记的蛋白质和高场光谱仪。**

Eight NMR instruments are housed in the Chemistry Department of McGill University and supports the training and research of more than 300 Chemistry HQPs per year in 32 different research groups in that Department. Additionally, there are many other HQPs and PIs from other departments at McGill and other academic institutions within Canada whose research requires the use of these NMR instruments. Therefore, the Helium Recovery Instrument requested by the five co-applicants of this NSERC RTI is submitted on behalf of all the users of the NMR instruments in the Chemistry Department of McGill University. The instrument requested is a necessary upgrade for a sustainable new era in NMR studies, and will allow the optimal use of the PIs' NSERC Discovery Grants for maximum research productivity and HQP training.******Examples of research applications from the five co-applicants include the general use of NMR for characterization of small molecules, characterization of biomolecular interactions with small ligands and mechanistic studies for understanding catalytic transformations. Arndtsen's research focuses on the development of new transition metal catalyzed reactions, which range from metal catalyzed, bond-forming chemistry, multicomponent coupling reactions, to C-H bond functionalizations. As such, his HQPs rely exclusively upon the use of NMR analysis for monitoring reactions and catalyst development. Gleason's research focuses on novel organocatalysts for a range of synthetic transformations, such as polyene cyclizations, oxy-Cope and cycloaddition chemistry. His studies include exploring the fundamental underlying mechanisms, which requires careful kinetic analysis, including 13C isotope experiments, that make extensive use of high-field NMR. Li's research focuses on unconventional and novel chemical transformations that can change the efficiency of chemical synthesis into more Green and Sustainable Chemistry. Each one of his research projects involves extensive liquid and solid-state NMR characterization of compounds and use of NMR for mechanistic studiers. Tsantrizos' research aims to exploit the molecular recognition elements affecting enzymatic and chemically-mediated catalytic processes. Her group is designing phosphorus-containing molecules that can be used as enzyme inhibitors and as ligands in metal-mediated and organocatalytic asymmetric synthesis. In the course of these investigations, NMR studies are required to elucidate the enzyme-bound conformation of ligands and catalyst-substrate interactions. Trempe's investigations focus on elucidating protein-protein interactions involving PINK1 and Parkin, two proteins that work together in a mitochondrial quality control pathway implicated in Parkinson's disease. His investigations require extensive NMR HSQC titration experiments using 15N- and 13C- labeled proteins and high-field spectrometers. **