Freeze-dryer console

我们的主要目标之一是开发稳健的合成方法，这些方法适合于高通量并行合成化合物，这些化合物将作为研究双分子相互作用复杂网络的工具。 目前，我们专注于杂环和肽模拟物支架的设计和合成，可以选择性地调节人法呢基焦磷酸合酶（hFPPS）在体外，在基于细胞的测定和动物模型中的催化活性。 hFPPS酶在甲羟戊酸途径的第一个关键步骤中占据战略位置，导致Ras致癌蛋白的蛋白质异戊二烯化。 此外，这种酶与干扰素诱导的人类先天性免疫应答有关。因此，hFPPS在宿主的代谢、肿瘤发生和先天免疫之间的交叉点起着至关重要的作用。这一领域的研究需要合成许多化合物，这些化合物必须通过反相HPLC纯化，然后冻干至干。 所要求的冻干机仪器是我们研究计划成功所需的一系列仪器中的关键组成部分。 这组仪器在实现化合物制备的效率和通量方面是必不可少的，所述化合物制备是为了彻底研究hFPPS与其活性位点抑制剂之间的相互作用，以及hFPPS与宿主因子蝰蛇蛋白之间的相互作用，在体外和基于细胞的生物测定中。

One of our key objectives is to develop robust synthetic methodologies that are amenable to high throughput parallel synthesis of compounds that will serve as tools in investigations of complex networks of bimolecular interactions. Currently, we are focusing on the design and synthesis of heterocyclic and peptidomimetic scaffolds that can selectively modulate the catalytic activity of human farnesyl pyrophosphate synthase (hFPPS) in vitro, in cell-based assays and in animal models. The hFPPS enzyme occupies a strategic location at the first committed step of the mevalonate pathway leading to protein prenylation of the Ras oncogenic proteins. In addition, this enzyme is implicated in the interferon-induced innate immune response against viral infections in humans. Therefore, hFPPS plays a crucial role at a cross-over point between the host's metabolism, oncogenesis and innate immunity. Studies in this area require the synthesis of numerous compounds which must be purifies by reversed phase HPLC and subsequently lyophilized to dryness. The requested Freeze-Dryer instrument is a key component within a cluster of instruments required for the success of our research program. This cluster of instruments is essential in achieving the efficiency and throughput of compound preparation that is needed in order to thoroughly investigate the interactions between hFPPS and its active site inhibitors, as well as the interactions between hFPPS and the host factor viperin, in vitro and in cell-based biological assays.