Electrochemical Aptamer-Based Biosensors for Salivary Molecular Measurements

开发一种适用于即时护理的设备，用于测量牙龈炎发病所固有的特定生物标志物的浓度，将改变目前临床上对这种疾病的诊断方式。同样，这项技术将允许患者在舒适的家中以类似于血糖仪的方式保持与他们口腔健康的联系。为此，我们建议与我们的工业合作伙伴合作，评估基于电化学适配体的生物传感器在未稀释唾液中的部署能力，这是一种非侵入性基质，用于监测患者的健康状况并测量与该疾病相关的固有生物标志物。基于电化学适配体的生物传感器已经展示了前所未有的能力，可以直接在未稀释的复杂基质（如全血）中进行实时、连续、单步和无试剂的大范围分子测量，甚至直接在活体中进行测量。不幸的是，目前还没有关于它们在未稀释的唾液中容易部署的能力的可行性研究，这反过来又限制了它们在临床和护理点的适用性。考虑到基于电化学适配体的生物传感器几乎可以检测到任何感兴趣的目标，通过改变适配体的简单权宜之计，它们可以证明在口腔健康环境中具有变革性，可以帮助提供者识别风险并优化预防。该提案旨在获得必要的资金，以进行模型系统的概念验证研究，以评估其对该传感平台分析性能的影响，并评估开发漂移和校准策略的必要性。电化学适体生物传感器部署在未稀释的唾液中。我们设想，这项可行性研究将证明在追求一个方便和通用的传感平台，以改善口腔健康至关重要。

Developing a device amenable to the point-of-care for the measurement of the concentrations of specific biomarkers that are inherent to the onset of gingivitis would transform how this disease is currently diagnosed in the clinic. This technology would, likewise, allow patients to remain connected to their oral health within the comfort of their homes in an analogous fashion to the glucometer. In response, we propose here, with the collaboration of our industrial partner, to evaluate the ability of electrochemical aptamer-based biosensors to readily deploy in undiluted saliva, a non-invasive matrix to monitor patients' health and measure inherent biomarkers of interest associated with this disease. Electrochemical aptamer-based biosensors have demonstrated unprecedented abilities in performing real-time, continuous, single-step and reagentless measurements of a wide range of molecules directly in undiluted complex matrices, like whole blood, and even directly in the living body. Unfortunately, no viability studies toward their ability to readily deploy in undiluted saliva have been completed, which in turn, limits their applicability in the clinic and at the point-of-care. Considering that electrochemical aptamer-based biosensors can detect virtually any target of interest, via the simple expedient of changing the aptamer, they could prove transformative in an oral health setting to help providers identify risks and optimize prevention. This proposal is for securing the necessary funds to undertake a proof-of-concept study of model systems electrochemical aptamer-based biosensors deployed in undiluted saliva to evaluate its impact on the analytical performance of this sensing platform and evaluate the need to develop drift and calibration strategies. We envision that this viability study will prove essential in the pursuit of a convenient and generalizable sensing platform to improve oral health.