Aptamer-based electrochemical sensor for ochratoxin

基于适体的赭曲霉毒素电化学传感器

• 批准号: 478783-2015
• 负责人: Sen, Dipankar
• 金额: $ 1.82万
• 依托单位: Simon Fraser University
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=579776
• 关键词: Aptamer; based; electrochemical; sensor; ochratoxin

Food safety is of paramount importance to the flourishing agriculture sector of Canada. It is a matter of great practical importance to develop cost-effective, portable, detection- and quantitation-capable sensors for undesirable contaminants such as fungus-derived mycotoxins within important crops such as grapes, grown for the Canadian wine-making industry, and fresh fruits such as cherries, intended for export all over the world. To date, conventional means for detection of mycotoxins in agricultural crops frequently makes use of expensive, time-consuming, and cumbersome antibody-based technologies. This NSERC ENGAGE proposal sets forth a short-term, initiating, collaboration between the laboratory of Dr. Dipankar Sen, at Simon Fraser University, and the Summerland, BC-based company, Flex Alert, that specializes in developing technologies for use in intelligent monitoring of fruit growth and packaging. Flex Alert has a long-term interest in the sensitive detection of a variety of mycotoxins produced by fungal infections of food crops. The proposed collaborative project between the Sen group/SFU and Flex Alert will focus on the design, synthesis, biochemical and electrochemical testing of a prototype Deoxyribosensor (DORS) device capable of detecting the mycotoxin, Ochratoxin A (OTA) in solution extracts from food crops. This project represents a propitious coming together of the DORS technology, invented by Dr. Sen and patented by SFU, an OTA-detecting aptamer assigned to Flex Alert, and Flex Alert's own business goals for creating an efficient new device for mycotoxin monitoring. Successful completion of this project will spur a deeper collaboration between SFU and Flex Alert; furthermore, the development of new and marketable mycotoxin detection devices will benefit farmers in both British Columbia and Canada as a whole.

食品安全对加拿大蓬勃发展的农业部门至关重要。这是一件大事 开发具有成本效益、便携、能够检测和定量的传感器的实际重要性 不受欢迎的污染物，如真菌衍生的真菌毒素，在葡萄等重要作物中种植用于 加拿大的酿酒业，以及樱桃等新鲜水果，打算出口到世界各地。 到目前为止，检测农作物中真菌毒素的常规方法经常使用 昂贵、耗时和繁琐的基于抗体的技术。 这项NSERC参与建议提出了一项短期的、启动的合作，由博士生博士的实验室进行。 西蒙·弗雷泽大学的Dipankar Sen和总部位于不列颠哥伦比亚省萨默兰的Flex Alert公司 专门开发用于水果生长和包装的智能监控技术。Flex Alert对灵敏地检测由真菌产生的各种真菌毒素有长期的兴趣 粮食作物的感染。Sen Group/SFU和Flex Alert之间拟议的协作项目将 重点研究了脱氧核糖传感器样机的设计、合成、生化和电化学测试 (DORS)能够检测粮食作物溶液提取物中的真菌毒素--赭曲霉毒素A(OTA)的设备。 这个项目代表了Dors技术的一个幸运的结合，该技术是由Sen博士和 由SFU获得专利，分配给Flex Alert的OTA检测适配子，以及Flex Alert自己的业务目标 创造了一种高效的真菌毒素监测新设备。这一项目的成功完成将刺激 SFU和Flex Alert之间更深入的协作；此外，开发新的适销对路的 真菌毒素检测设备将使不列颠哥伦比亚省和加拿大的农民作为一个整体受益。