2-ZYME: Two-step biocatalytic conversion of underused biorefinery side-streams to glucaric acid

2-ZYME技术应用生物催化转化的选择性，将主要生物资源部门未充分利用的生物质馏分转化为抢手的生物化学品。通过利用未充分利用的生物质中的自然结构，2-ZYME技术提高了生物资源效率，同时使现有生物精炼厂的产品组合多样化。该技术将两种酶结合在一个锅中，分别从木材和农业生物精炼厂的未充分利用的部分葡萄糖醛酸和生物乙醇中生产葡萄糖酸。葡萄糖酸是一种平台化学物质，是生物聚合物合成的关键组成部分，也被用于无磷洗涤剂和除冰剂中。2016年全球葡萄糖酸市场规模估计为5.504亿美元，预计2018-2023年期间的累积平均增长率（CAGR）为7%。与传统方法相比，2-ZYME技术的优点是产品纯度高，产率高，原料成本低。最近完成的NSERC I2I市场评估2-ZYME技术证实了这种新酶系统的市场准备。目前的NSERC I2I第1阶段项目将侧重于确保私营部门投资所需的三个具体任务：1)对现有生物精炼厂生产的未充分利用的生物质流进行详细的成分分析，为部署工作提供信息；2)将2- zyme技术应用于优先的葡萄糖二氧胺来源；3)证明2- zyme技术的实验室规模扩大，包括产品回收。为了实现项目目标，提议的行动集合了承诺的工业合作伙伴，包括原料供应商（Fortress Advanced Bioproducts, IGPC Ethanol），生物技术开发商（Novozymes）和市场专家（Pansophy Consulting），以创建一个专注的学术-行业合作伙伴关系，该合作伙伴有动力并充分准备实现2-ZYME技术的经济潜力和环境效益。

The 2-ZYME technology applies the selectivity of bio-catalytic conversions to transform underused biomass fractions of major bioresource sectors into sought-after biochemicals. By leveraging natural structures in underused biomass, the 2-ZYME technology increases bioresource efficiency while diversifying the product portfolio of existing biorefineries.The technology combines two enzymes in one pot to produce glucaric acid from glucuronoxylans an underused fraction of wood and agricultural biorefineries making cellulose pulps and bioethanol, respectively. Glucaric acid is a platform chemical that serves as a key building block for biopolymer synthesis, and is also used as such in phosphate-free detergents along with de-icing agents. The global glucaric acid market size was estimated at USD 550.4 million in 2016 with a projected cumulative average growth rate (CAGR) of 7% during the 2018-2023 period. Advantages of the 2-ZYME technology over the conventional approach to producing glucaric acid include high product purity and yield, and low feedstock costs.A recently completed NSERC I2I Market Assessment of the 2-ZYME technology confirmed the market readiness for this new enzyme system. The current NSERC I2I Phase I project will focus on three specific tasks needed to secure private sector investment: 1) detailed compositional analysis of underused biomass streams produced by existing biorefineries to inform deployment efforts, 2) application of the 2-ZYME technology to the prioritized glucuronoxylan sources, and 3) prove out lab scale-up of the 2-ZYME technology including product recovery.To achieve the project goals, the proposed action assembles committed industrial partners, including feedstock providers (Fortress Advanced Bioproducts, IGPC Ethanol), biotechnology developers (Novozymes), and market experts (Pansophy Consulting), to create a focused academic-industry partnership that is motivated and fully equipped to realize the economic potential and environmental benefits of the 2-ZYME technology.