Biomolecular Self-Assembly of Hydrophobins and Fusion Constructs for Biocatalytic Surfaces and Multi-enzyme Complexes

疏水蛋白的生物分子自组装以及生物催化表面和多酶复合物的融合结构

• 批准号: 327470-2012
• 负责人: Master, Emma
• 金额: $ 2.48万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=572029
• 关键词: Biomolecular; Self; Assembly; Hydrophobins; Fusion

Hydrophobins are among the most surface-active molecules known. The amphiphilic properties of hydrophobins promote their self-assembly into films at the interface of liquid and air, liquid and solids, and between liquid phases. This unique family of small amphiphilic proteins are expressed by filamentous fungi and are thought to modulate cell adhesion to solid substrates and mycelial surface tension. The hydrophobin family is subdivided into two classes, I and II, based on the hydropathy pattern and solution behavior of corresponding proteins. While Class II hydrophobins are solubilized in alcohol-detergent mixtures and form films characterized by ordered monolayer morphology, class I hydrophobins are unique in their ability to form highly stable aggregates and interfacial films characterized by a regular rodlet morphology. Notably, solution aggregates of class I hydrophobins are reminiscent of amyloid-like fibrils that characterize the pathogenesis of neurological diseases including Alzheimer's. To date, hydrophobins have been developed as emulsifiers, foam stabilizers, water-repelling coatings and as protein purification tags. In these cases, mainly two hydrophobins have been employed. The overall aim of the proposed research program is to investigate and compare the self-assembly of diverse hydrophobin proteins, and to determine how this bioprocess is influenced by environmental parameters and enzyme fusion design. In this way, our aim is to advance the potential of hydrophobins to enhance synthetic enzyme catalyses at liquid-liquid interfaces, create novel multi-enzyme complexes that combine multi-step or synergistic enzyme activities, and develop biocatalytic coatings for plant biomaterials.

疏水蛋白是已知的最具表面活性的分子之一。疏水蛋白的两亲性促进了它们在液体与空气、液体与固体以及液相之间的界面处自组装成膜。 这种独特的小两亲性蛋白家族由丝状真菌表达，并且被认为调节细胞对固体基质的粘附和菌丝体表面张力。 疏水蛋白家族基于相应蛋白质的亲水性模式和溶液行为被细分为两类，I和II。虽然II类疏水蛋白溶解于醇-洗涤剂混合物中并形成以有序单层形态为特征的膜，但I类疏水蛋白在形成以规则棒状形态为特征的高度稳定的聚集体和界面膜的能力方面是独特的。值得注意的是，I类疏水蛋白的溶液聚集体使人联想到淀粉样蛋白样原纤维，其表征包括阿尔茨海默氏症的神经疾病的发病机制。 迄今为止，疏水蛋白已被开发为乳化剂、泡沫稳定剂、防水涂层和蛋白质纯化标签。在这些情况下，主要使用两种疏水蛋白。 拟议的研究计划的总体目标是调查和比较不同的疏水蛋白的自组装，并确定这种生物过程是如何受到环境参数和酶融合设计的影响。 通过这种方式，我们的目标是提高疏水蛋白的潜力，以增强合成酶催化在液-液界面，创造新的多酶复合物，联合收割机多步骤或协同酶的活动，并开发植物生物材料的生物催化涂层。