Market assessment proposal for Animal Health Rapid Diagnostic

目前的动物疾病诊断市场包括两大类测试：分子测试，如PCR测试，必须在设备齐全的实验室中由训练有素的人员完成，以及快速测试，如侧向流抗原测试，可以由用户进行培训，但已知是不可靠的。认识到这一挑战的重要性，我们采用合成生物学方法开发了一种分子测试，由基于基因电路的传感器和无细胞表达系统（CFS）组成，其读数可以使用现成的血糖仪1进行解释。血糖仪在糖尿病管理中的广泛应用已经形成了全球设备制造和分销网络，并被患者和临床医生广泛接受，可供受过培训或未接受过培训的用户使用。我们最近展示了该技术用于检测多种疾病1，包括影响家禽2和猪3的甲型副伤寒和B，以及COVID-19。我们正在将我们的技术应用于动物疾病诊断，目标是将临床级的分子检测交到兽医和农场管理人员手中。这是一个独特的机会，为伴侣动物、马或食用动物健康市场的重要动物疾病提供更低成本、更可靠的诊断测试。我们正在申请I2 I市场评估资助，目的是接触行业和潜在客户，以确定客户需求和目标市场。这将有助于告知和阐明我们的价值主张，这将使我们能够优化我们的市场进入战略，以成功地将我们的技术商业化，并为我们的研发工作提供重点，包括选择新的疾病目标。Amalfitano，E.，Karlikow，M.，Norouzi，M.等人，Nat Commun 12，724（2021）。Shivaprasad，H. L. Rev Sci Tech. Aug;19（2）：405-24（2000）。Patterson，S. K.等人，Anim Health Res Rev. Dec;17（2）：159-168（2016）。

The current market for animal disease diagnostics consists of two key categories of tests: molecular tests, such as the PCR test that must be completed in a fully equipped lab with trained personnel, and rapid tests such as lateral flow antigen tests, which can be performed by users with little training but are known to be unreliable. Recognizing the importance of this challenge, we employed synthetic biology methods to develop a molecular test, consisting of gene circuit-based sensors and cell free expression system (CFS), with a readout that can be interpreted using an off-the shelf blood glucose meter1. The widespread adoption of the glucose meter for diabetes management has resulted in a global network of device manufacturing and distribution, as well as broad acceptance by patients and clinicians for use by trained or naive users.We have recently demonstrated this technology for the detection of several diseases1, including paratyphoid A and B, which affect poultry2 and swine3, and COVID-19. We are adapting our technology to animal disease diagnostics, with the goal to put clinical-grade molecular tests into the hands of veterinarians and farm managers. This is a unique opportunity to provide lower-cost, more reliable diagnostic tests for important animal diseases in the companion animal, equine, or food animal health markets.We are applying to the I2I Market Assessment grant with the goal of reaching out to the industry and potential customers to identify customer needs and target markets. This will help inform and articulate our value proposition, which will allow us to optimize our market entry strategy for successful commercialization of our technology, as well as provide focus for our research and development efforts including selection of new disease targets.1. Amalfitano, E., Karlikow, M., Norouzi, M. et al.Nat Commun 12, 724 (2021).2. Shivaprasad, H. L. Rev Sci Tech. Aug;19(2):405-24 (2000).3. Patterson, S. K. et al. Anim Health Res Rev. Dec;17(2):159-168 (2016).