There's something in the air: disentangling the role of airborne microbes and pollutants in the inflammatory response of asthma along a latitudinal gradient in Canada

哮喘是一种炎症性呼吸道疾病，影响超过380万加拿大人，其中约四分之一是儿童。很少有研究调查了北方加拿大的哮喘发病率，但因纽特儿童的哮喘发病率低于其他土著和非土著儿童。这很有趣，因为哮喘不仅受遗传因素的影响，还受环境因素的影响。因此，一个人生活在哪里对患哮喘的风险及其严重程度有重大影响。这可能是由于暴露于过敏原，也可能是由于其他保护性或炎症过程。例如，最近的研究发现，在具有不同微生物群落的地区（如农田）长大可以通过帮助人类相关微生物（微生物组）训练免疫系统来降低患哮喘的可能性。更直接地，已经发现某些空气中的微生物通过防止气道的严重炎症而对哮喘的发展具有保护作用。微生物并不是空气质量的唯一方面，可以导致哮喘。与资源开采、运输以及消费品和商业产品相关的污染物可能会产生相反的效果，并引起气道炎症和氧化损伤，从而导致症状的严重程度。因此，空气中的环境微生物和污染物对哮喘有相反的影响，这些因素对一个人的居住地具有高度特异性。然而，目前还不清楚这两个变量如何交叉，以及它们如何共同促进或阻碍哮喘的表现。我们的目标是研究如何空气质量可能有助于炎症和哮喘在体外共培养细胞模型的人类呼吸道。我们将沿着人口规模、植被和土地用途：不同的梯度对4个地区进行沿着采样：斯卡伯勒（安大略，人口~ 632 k）、萨格奈（魁北克，~ 69 k）、库朱阿拉皮克（努纳维克，~686）和雷索卢特湾（努纳武特，~198）。在所有地点，我们将测量空气污染物（挥发性有机化合物，微量元素，颗粒物），花粉和微生物群落，通过宏基因组测序进行表征。空气中的微生物也将从每个地点收集和浓缩。在实验室中，我们将人类呼吸道的共培养细胞模型暴露于这些现场微生物。共培养物还将暴露于不同浓度的污染物，代表我们的现场数据。然后，我们将测量氧化应激和炎症的指标，以量化微生物和污染物在哮喘中的作用，并确定保护免受炎症的生物体。我们假设环境中空气微生物的多样性将在我们的北方站点中产生保护作用，该站点在靠近树木线处减少。我们还预计代表我们北方研究中心的空气污染物水平将导致更少的炎症。这项研究提出了一种新的方法来了解环境如何塑造哮喘的病因。我们的机械方法将使我们能够解开呼吸道炎症的主要环境变量的作用，并确定哮喘的驱动因素沿着加拿大的纬度梯度。

Asthma is an inflammatory respiratory disease that affects over 3.8 million Canadians, approximately a quarter of which are children. Few studies have investigated the morbidity of asthma in Northern Canada, but Inuit children have been found to have lower prevalence of asthma compared to other Indigenous and non-Indigenous children. This is interesting because asthma is not only influenced by genetics but also by environmental factors. Therefore, where an individual lives has a significant impact on the risk of developing asthma and its severity. This can be due to exposure to allergens but also to other protective or inflammatory processes. For example, recent studies have found that growing up in areas with diverse microbial communities such as farmland can decrease the likelihood of developing asthma, by helping human-associated microbes (the microbiome) train the immune system. More directly, certain airborne microorganisms have been found to have a protective role against the development of asthma by preventing serious inflammation of the airways. Microorganisms are not the only aspect of air quality that can contribute to asthma. Pollutants associated with resource extraction, transportation, as well as consumer and commercial products can have the opposite effect and induce inflammation and oxidative damage to the airways thus contributing to severity of symptoms. Airborne environmental microbes and pollutants therefore have opposite effects in asthma, and these factors are highly specific to where a person lives. However, it is unclear how these two variables intersect, and how together they may promote or impede the presentation of asthma. Our goal is to investigate how air quality may contribute to inflammation and asthma in an in vitro co-culture cell model of the human respiratory tract. We will sample 4 regions along a gradient varying in population size, but also in vegetation and land use: Scarborough (Ontario, population ~632k), Saguenay (Quebec, ~69k), Kuujjuarapik (Nunavik, ~686) and Resolute Bay (Nunavut, ~198). At all sites, we will measure air pollutants (volatile organic compounds, trace elements, particulate matter), pollen, and microbial communities for characterization through metagenomic sequencing. Airborne microbes will also be collected and concentrated from each site. In the lab, we will expose a co-culture cellular model of the human respiratory airway to these field microbes. The co-culture will also be exposed to varying concentrations of pollutants, representative of our field data. We will then measure indicators of oxidative stress and inflammation, to quantify the role of both microbes and pollutants in asthma and identify organisms that protect from inflammation. We hypothesize that the diversity of airborne microbes in an environment will have a protective effect in our northern sites, which decreases closer to the treeline. We also expect airborne pollutant levels representative of our Northern sites to induce less inflammation. This study proposes to use a novel approach to understand how the environment shapes the etiology of asthma. Our mechanistic approach will allow us to disentangle the role of the major environmental variables in inflammation of respiratory airways, and to identify the drivers of asthma along a latitudinal gradient in Canada.