Critical Use UVvis Spectrophotometer

这笔资金申请用于更换和升级远远超过其使用寿命的过时仪器。哈德逊集团自1999年以来一直使用瓦里安Cary 300 Bio，在一系列其他严重问题（主板和光学列车）后（3年前）遭受了Peltier设备的灾难性故障，这些问题近年来以不菲的费用进行了维修，但不再能够修复。因此，我们不再有足够的仪器来进行我们的研究。在过去的22年里，目前的仪器对我们的研究任务至关重要。所要求的仪器将主要服务于Hudson组（平均组：4个研究生，2个UG）；Konermann组（平均组：8名研究生，2名本科生）和Workentin组（平均组：6名研究生，2名本科生）。与我们以前的仪器一样，我们期待并欢迎Wisner组（2-3名学生，用于表征光开关分子），Luyt组（7-8名学生，与我们的PTI荧光光谱仪结合进行量子产率计算）的定期使用和偶尔使用。此外，具有扩展红色范围的新仪器可用于金纳米团簇和有机和无机荧光团（Gilroy小组，11名学生）。瓦里安Cary 300仪器已被安捷伦Cary 3500所取代，后者具有显着改进的设计，通过由peltier装置和多光束设计控制温度的固定八个位置样品室取代了容易出错的机械传输。此外，重新设计的仪器使用氙灯，而不是Cary 300的双氘/可见光灯设计，后者更坚固，更经济，能够快速收集数据，用于研究结合相互作用的动力学。该仪器还具有风冷式Peltier加热/冷却块，无需外部浴槽，这也消除了故障点。该仪器具有灵敏度和光度稳定性，适合于研究熔体和还原曲线通常需要的长时间实验。此外，具有细胞内温度监测的快速温度上升是可用的-这一功能将被用于开发改进的方法，以更节省时间的方式确定核酸模拟物的热稳定性。

This funding request is for the replacement and upgrade of obsolete instrumentation that is well beyond its serviceable lifetime. The Hudson group has used a Varian Cary 300 Bio since 1999 which suffered a catastrophic failure of the Peltier device (3 years ago) after a litany of other serious problems (motherboard and optical train) which were serviced in recent years at no small expense, but is no longer able to be repaired.  As such, we no longer have adequate access to instrumentation to pursue our research. The current instrument has been critical to our research mission for the past 22 years. The requested instrument will primarily service the Hudson group (average group: 4 graduate, 2 UG); Konermann group (average group: 8 graduate, 2 undergraduate) and the Workentin group (average group: 6 graduate, 2 UG). As with our previous instrument, we expect and welcome regular use from the Wisner group (2-3 students, for characterization of photo-switchable molecules), the Luyt group (7-8 students, for quantum yield calculation in conjugation with our PTI spectrofluorimeter) and occasional use from groups. As well, the new instrument with extended red-range useful for gold-nanoclusters and organic and inorganic fluorophores (Gilroy group, 11 students).  The Varian Cary 300 instrument has been superseded by the Agilent Cary 3500 which possesses significantly improved design, by replacement of the error-prone mechanical transport by a fixed eight-position sample chamber that is temperature controlled by a Peltier-device and a multi-beam design. Additionally, the re-designed instrument uses a xenon lamp instead of the dual deuterium/visible lamp design of the Cary 300 which is more robust, economical and is capable of fast data collection for use in studying the kinetics of binding interactions. The instrument also features an air-cooled Peltier heating/cooling block eliminating the need for an external bath, which is also a point of failure eliminated. The instrument has the sensitivity and photometric stability suitable for the long experiments typically needed to study melts and renaturation curves. Additionally, rapid temperature ramping, with in-cell temperature monitoring, is available - this feature will be exploited to develop improved methodologies for determining nucleic acid analog thermal stabilities in a more time-economical fashion.