Microplate based LSPR biosensors

生物分子之间的相互作用是所有生物化学过程的基础，因此是治疗学和生物技术的核心。因此，了解这些相互作用的优势是开发新技术的基础。无标签技术，如局部表面等离子体共振（LSPR）是测量这些重要相互作用的金标准，但由于昂贵的专用光学设备，LSPR仪器的使用受到限制。目前的商用传感器还受到非特异性相互作用的限制，不能轻易地在血液等复杂环境中使用，这进一步阻碍了实用性。为了克服这项重要技术的这两个主要限制，我们开发了一种用于板阅读器的新型传感器，这是一种常见的实验室设备，它使用可扩展的技术来生产减少非特异性相互作用的涂层。到目前为止，这个新的和可访问的LSPR平台已经引起了业界领先的车牌阅读器制造商和治疗开发公司的关注。该提案包括一个工作计划，将我们的概念验证传感器转化为一个自动化流体处理平台，该平台与普通板读取器兼容，并扩展涂层的化学性质，使传感器能够用于与治疗开发相关的更广泛的分析目标。在I2I项目期结束时，我们的目标是开发一个可供治疗开发人员使用的工作平台，并获得制造合作伙伴的许可。

Interactions between biomolecules underlie all biochemical processes and so are central to therapeutics and biotechnology. Understanding the strengths of these interactions is thus fundamental to the development of new technologies. Label-free techniques such as local surface plasmon resonance (LSPR) are the gold standard for measuring these important interactions, but access to LSPR instrumentation is limited due to expensive, specialized optical equipment. Current commercial sensors are also limited by non-specific interactions, and can't be easily used in complex environments such as blood, which further hinders utility. To overcome these two main limitations of this important technique, we have developed a new type of sensor for use in a plate reader, which is a common piece of laboratory equipment, and which uses a scalable technique to produce a coating that reduces the non-specific interactions. So far, this new and accessible LSPR platform has garnered attention from both industry leading plate reader manufacturers and therapeutic development companies. This proposal includes a work plan that will translate our proof-of-concept sensors into an automated fluid handling platform which is compatible with common plate readers and expand the chemistry of the coatings to allow the sensors to be used with a broader range of analytical targets relevant to therapeutic development. By the end of this I2I project period, we aim to develop a working platform that can be used by therapeutic developers and licensed with a manufacturing partner.