基于聚集诱导发光分子功能化金纳米粒子的比色/荧光同步响应检测方法的研究

因其独特的荧光性质，聚集诱导发光分子越来越受到人们重视，成为目前分析化学一个新兴研究领域。新型聚集诱导发光聚集体的开发是研究聚集诱导发光机理、建立新颖分析方法的重要途径。近年来，基于聚集变色原理，纳米金比色检测方法在分析化学领域得到广泛应用。本项目拟将聚集诱导发光分子共价联接到纳米金表面，建立新型聚集诱导发光纳米复合物，将纳米金“聚集变色”的性质与聚集诱导发光分子“聚集增强荧光”的特性结合，实现比色法和荧光两种检测方法有机统一。项目将研究聚集诱导发光分子在纳米金表面的光学性质,并将此类新型聚集诱导发光复合物应用于分析检测领域：基于某些特异性作用，新型聚集诱导发光复合物与目标分子作用以后，分散性发生改变，从而使其紫外-可见吸收光谱与荧光光谱随之变化，据此建立一些简便、快速、高灵敏度、比色法与荧光同步响应的检测方法。项目的开展有望深入探讨聚集诱导发光机理，拓展聚集诱导发光应用领域。

The study of aggregation induced emission(AIE) molecules is attracted more and more attentions and becomes hot research area in analytical chemistry due to its unique fluorescent property. The development of new type AIE aggregates is an important way to reveal the mechanism of AIE and build novel analytical method. Au nanoparticles (Au NPs) have been widely used in the development of colorimetric detection method based on its property of aggregation induced color change．In this project, AIE molecules will be attached to the surface of Au NPs by covalent bond, forming new type AIE nano-composites (named AIE/Au NPs)with colorimetric and fluorescent synchronous response property through the combination of aggregation induced emission from AIE molecule with aggregation induced color change from AuNPs. And then, the optical properties of AIE/Au NPs will be investigated in the absence or presence of target molecules. The UV-visible spectra and fluorescent spectra of AIE/Au NPs will be changed in the presence of target molecule due to the alteration of dispersibility of AIE/Au NPs. Thus, some facile, fast response and high sensitivity with colorimetric and fluorescent synchronous response methods will be developed. This study can be helpful to investigate the mechanism of AIE and expand related application.