纳米载体高效递送dsRNA系统的构建及毒理学效应研究

RNA干扰（RNAi）被广泛应用于功能基因和功能基因组研究，且近年来聚焦RNAi靶向抑制有害生物相关蛋白的翻译、合成及创制RNAi农药用于有害生物防治。但高效递送靶标dsRNA方法的缺乏，极大的限制了RNAi农药创制与应用。基于此，本项目拟制备层状双氢氧化物等纳米材料为dsRNA高效递送载体，以二化螟高致死率的特异性dsCs-Cht4等为模式dsRNA，制备LDHs-dsRNA，以水稻二化螟为靶标害虫，评价LDHs-dsRNA的生物活性和持效期，揭示LDHs-dsRNA的浓度与生物活性、持效期的关系，阐明LDHs-dsRNA增强dsRNA在鳞翅目昆虫中RNAi效率的分子机理。预期研究结果有助于提升RNAi在昆虫功能基因和功能基因组的研究效率及为RNAi农药创制提供理论依据，亦可为农业害虫的防治提供新策略。

RNA interference (RNAi) is widely used in functional gene and functional genomics research. In recent years, scientists focus on RNAi targeted inhibition of pest related protein translation and synthesis and creation of RNAi pesticides for pest control. However, the lack of efficient delivery of target dsRNA methods has greatly limited the creation and application of RNAi pesticides. Based on this, nanomaterials such as layered double hydroxide is prepared as a high-efficiency dsRNA delivery carrier. High lethality specific dsRNA to Chilo suppressalis such as dsCs-Cht4 is selected as the model dsRNA. LDH-dsRNA is proposed to prepared. C. suppressalis is used as the target pest to evaluate the biological activity and duration of LDHs-dsRNA to C. suppressalis. The relationship between the concentration of LDHs-dsRNA and biological activity and duration will be revealed. The molecular mechanism of RNAi efficency increased by LDHs-dsRNA in Lepidoptera will be elucidated. The expected results will help to improve the efficiency of RNAi research on insect functional genes and functional genomes, provide a theoretical basis for the creation of RNAi pesticides, as well as new strategies for the prevention and control of agricultural pests.