茶树菇Agrocybe aegerita中活性蛋白/肽的系统鉴定和功能研究

近年的研究表明，多种食品中的活性肽具有多种生物活性，是食品生物化学的研究重点和热点领域。食用菌中含有丰富的肽，长期以来被当做普通的营养成分。本人通过博士期间研究，发现食用菌茶树菇（A.aegerita）中肽成分具有抗肿瘤活性，从中鉴定了具有抗肿瘤活性的3种茶树菇活性肽。本项目将对茶树菇中的活性肽成分进行系统鉴定，通过层析技术分离纯化茶树菇天然肽，以及酶解子实体蛋白制备系列肽库，并利用膜分离技术和高效液相色谱分离纯化，利用质谱技术鉴定序列，系统评价天然肽和酶解肽的抗肿瘤活性。本项目将建立茶树菇的活性肽数据库，鉴定有自主知识产权的活性肽，为科学开发食用菌活性肽提供理论依据。

Bioactive peptides derived from food exert a wide range of nutritional, functional and biological activities and have become a focus of food biochemistry research. Edible fungi are rich in peptides which are regarded as nutritional components. From my PhD research, I have found that the peptides of A.aegerita have antitumor activity. We have purified and identified 3 antitumor peptides from it. This project will study on systematic identification and function of bioactive peptides in A.aegerita.We will separate natural peptides in A.aegerita by chromatography. On the other hand, peptide pools will be prepared by enzymatic hydrolysis. Then the peptides will be separated and sequenced by HPLC-MS/MS. The antitumor activities of natural peptides and peptides produced from enzymolysis of proteins will be scored by bioactive function examination. This research can not only built up a database of bioactive peptides of A.aegerita and identify bioactive peptides with independent intellectual property rights, but also provide theoretial support for development of bioactive peptides in edible mushroom.