苗药黑骨藤在不同生理状态下的药动学及代谢特征研究

黑骨藤在贵州苗族地区广泛以内服外用治疗风湿关节痛、跌扑损伤，为我省十大苗药之一。课题组首次研究发现黑骨藤水溶性有效组分治疗类风湿性关节炎疗效独特，但其药效物质基础、作用机制及体内过程尚不明确。本研究旨在以黑骨藤有效组分为研究对象，（1）开展黑骨藤在正常大鼠和病理模型大鼠的体内移行成分及谱效关系研究，分析其特征差异以及与抗炎活性的相关性；（2）建立黑骨藤PK-PD模型，分析正常和病理状态下药代动力学与药效动力学的相关性，揭示其抗炎作用的效应成分及体内动态变化规律；（3）利用代谢组学方法，探讨黑骨藤对炎症动物模型代谢网络的影响，确定其对机体内源性代谢物及代谢通路的调控机制。上述研究结果将为阐明黑骨藤的药效物质基础和作用机制提供科学依据。

Periploca Forrestii, one of the ten key Miao’s medicinal plans in Guizhou province, is widely used for the treatment of rheumatic joint pain and traumatic injury in Guizhou ethnic minority regions. The investigation of our research team has proved for the first time that the active water-soluble ingredients in Periploca Forrestii had outstanding curative effect on rheumatoid arthritis (RA) while the material base for efficacy, action mechanism and process in vivo still remain unclear. The studies are planned to be conducted focusing on the effective ingredients of Periploca Forrestii . The following research work will be involved: (1) to conduct the study on the transitional constituents and fingerprint pharmacodynamics between the control group and animal pathological model group, then to treat the data and manage to the find out the relationship between the characteristic constituents and the anti-inflammatory activity and to identify the PK markers. (2) to build up the PK-PD models in rats and to discover the PK-PD relation under physiological and pathological conditions in order to reveal the law of anti-inflammatory action mechanism and the dynamic process in vivo of effective ingredients in Periploca Forrestii. (3) to study the effects on the metabolism network of model rats by metabolomics approach so as to discover the regulation mechanism of related endogenesis substance and metabolism path. Above research results will provide the evidence for the effective ingredients and mechanism of action of Periploca Forrestii.