傣药莫哈蒿干预特应性皮炎药效物质基础及调控ILC2-MC轴的作用机制的研究

特应性皮炎是一种免疫性皮肤病，在世界范围内呈上升的趋势，而临床上缺乏治疗该病的有效、安全的药物。最新研究表明，在AD的发病机制中，ILC2和MC存在直接的相互调控关系，可共同导致AD的炎症发展。申请人已经从民族药莫哈蒿中分离得到部分生物碱类化合物，并发现其中鸭嘴花碱能够明显降低“ILC2-MC”轴相关因子（IL-33、TSLP、IL-4、IL-13、IgE）的水平，故此推测莫哈蒿中具有通过作用于调控IL2上游炎性细胞因子（IL-33、TSLP）水平→作用于ILC2-MC细胞轴→调控Th2炎性细胞因子（IL-13、IL-4、IL-13）→抑制炎症发生而治疗AD的物质基础。本项目组拟从莫哈蒿中寻找和分离新的活性化合物，并以ILC2-MC 细胞轴为核心，综合运用流式细胞术、多光谱组织成像等多种技术方法研究莫哈蒿中化合物干预AD的作用机理，并通过体内外实体内外实验验证筛选出活性较强的先导化合物。

Atopic dermatitis is an immune dermatosis, which is on the rise worldwide. However, there is a lack of effective and safe drugs to treat AD. Recent studies have shown that in the pathogenesis of AD, there is a direct relationship between ILC2 and MC, which can lead to the development of inflammation in AD. The applicant has isolated some alkaloids from the national drug Adhatoda Vasica and found that vasicine can significantly reduce the levels of ILC2-MC axis-related factors (IL-33, TSLP, IL-4, IL-13, IgE). Therefore, it is speculated that Adhatoda Vasica has the function of regulating the levels of inflammatory cytokines (IL-33, TSLP) upstream of IL2 acting on the ILC2-MC axis regulating Th2 cell axis. Inflammatory cytokines (IL-13, IL-4, IL-13) Material basis for the treatment of AD by inhibiting inflammation. The project team intends to find and isolate new active compounds from Adhatoda Vasica. The ILC2-MC cell axis is taken as the core. Flow cytometry, multi-spectral tissue imaging, MSD, Western Blot and other techniques are used to study the mechanism of the intervention of compounds in Adhatoda Vasica on AD. The pilot compounds with strong activity are screened through in vivo and in vitro experiments. This study not only provides new ideas for searching for active lead compounds for the treatment of atopic dermatitis, but also provides scientific basis and technical support for the development and utilization of Yunnan national medicinal plant resources.