A rare case of intracytoplasmic mucin‐rich nasopharyngeal carcinoma
A rare case of intracytoplasmic mucin‐rich nasopharyngeal carcinoma
复制标题
胞浆内富含粘蛋白鼻咽癌一例罕见病例
DOI：
10.1002/dc.24934
复制
发表时间：
2022
影响因子：
1.3
通讯作者：
Kondo Tetsuo
中科院分区：
文献类型：
--
作者：
Yamaguchi Yohei;Odate Toru;Nakazawa Kumiko;Oishi Naoki;Mochizuki Kunio;Shimizu Tatsuya;Horiuchi Kiwako;Ishii Hiroki;Sakurai Daiju;Kondo Tetsuo
鼻咽癌是一种与EB病毒(EBV)相关的肿瘤，在亚洲国家有较高的发病率。鼻咽癌是一种起源于鼻咽粘膜的鳞状细胞癌。虽然罕见，但鼻咽癌表现出一些不常见的组织学变异；这些变异仍有待了解。我们描述了一种罕见的鼻咽癌变异体的细胞学特征，并伴有丰富的胞浆粘蛋白。一位37岁的日本男性因双侧耳部不适和可触及的淋巴结节而来本院就诊。鼻咽活检显示肿瘤细胞胞浆内有丰富的阿尔新蓝PAS阳性粘蛋白。免疫组织化学分析显示，肿瘤细胞p40和p53均为阳性。原位杂交(ISH)的EB病毒编码区(EBER)显示肿瘤细胞内有EBV感染。使用MAML2裂解探针的荧光原位杂交(FISH)未显示分裂信号。同时对转移的淋巴结进行细针吸取细胞学检查。涂片样本有坏死和炎症背景，淋巴细胞和中性粒细胞均有浸润。肿瘤细胞高度聚集，细胞散在，胞核裸露。肿瘤细胞胞质清晰，细胞边界清楚。液基细胞学标本中散在的肿瘤细胞中可观察到胞浆内粘蛋白将细胞核推向外围。根据这些发现，最终诊断为晚期鼻咽癌；以顺铂为基础的放化疗作为一线治疗。治疗结束后8个 月肿瘤复发。复发性鼻咽癌为典型的非角化性鼻咽癌，胞浆内缺乏粘蛋白。
Nasopharyngeal carcinoma (NPC) is an Epstein–Barr virus (EBV)‐associated tumor with a high incidence in Asian countries. NPC is a type of squamous cell carcinoma originating from the nasopharyngeal mucosa. Although rare, NPCs show some uncommon histologic variants; these variations remain to be understood. We described the cytologic characteristics of a rare NPC variant with abundant intracytoplasmic mucin. A 37‐year‐old Japanese man presented to our hospital with bilateral ear discomfort and palpable lymph nodes. Nasopharyngeal biopsy showed tumor cells with abundant intracytoplasmic, Alcian blue‐PAS‐positive mucin. Immunohistochemical analysis demonstrated that the tumor cells were positive for p40 and p53. Epstein–Barr encoding region (EBER)in situhybridization (ISH) showed EBV infection of the tumor cells. Fluorescencein situhybridization (FISH) using MAML2 break‐apart probes did not show split signals. Fine needle aspiration biopsy (FNAB) cytology of the metastatic lymph nodes was also performed. Smear samples had a necrotic and inflammatory background with both lymphocyte and neutrophil infiltration. Highly cellular tumor clusters and dispersed cells with naked nuclei were observed. The tumor cells showed a clear cytoplasm with distinct cell borders. Intracytoplasmic mucin pushing the nucleus to the periphery was observed in the scattered tumor cells in a liquid‐based cytology sample. Given these findings, the final diagnosis was advanced nasopharyngeal carcinoma; cisplatin‐based chemoradiation therapy was performed as the first‐line treatment. The tumor recurred 8 months after completing the treatment. The recurrent nasopharyngeal tumor was a typical non‐keratinizing NPC and lacked intracytoplasmic mucin.
登录
查看更多内容
日本原发性鼻咽粘液表皮样癌：两例组织化学和免疫组织化学分析及文献综述
影响因子：
3.5
作者：
K. Kusafuka;Y. Takizawa;Y. Iida;M. Ebihara;T. Onitsuka;T. Kameya
通讯作者：
T. Kameya
DOI：
10.4324/9780203306123_classification_of_head_and_neck_tumours
发表时间：
1998
期刊：
--
影响因子：
--
作者：
K. Riden;Present address
通讯作者：
K. Riden;Present address
鼻咽癌EB病毒RNA检测及腺体分化：附2例报告。
影响因子：
4.6
作者：
D. Jain;V. Parkash;M. Li;J. Gill;J. Crouch;G. Howe;G. Tallini
通讯作者：
G. Tallini
影响因子：
1.3
作者：
Kollur, SM;El Hag, IA
通讯作者：
El Hag, IA
影响因子：
2.1
作者：
F. Petersson
通讯作者：
F. Petersson