Some lumbar sympathetic neurons develop a glutamatergic phenotype after peripheral axotomy with a note on VGLUT₂-positive perineuronal baskets.
Some lumbar sympathetic neurons develop a glutamatergic phenotype after peripheral axotomy with a note on VGLUT₂-positive perineuronal baskets.
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DOI：
10.1016/j.expneurol.2011.05.004
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发表时间：
2011-08
影响因子：
5.3
通讯作者：
Gebhart, G. F.
中科院分区：
文献类型：
--
作者：
Brumovsky, Pablo R.;Seroogy, Kim B.;Lundgren, Kerstin H.;Watanabe, Masahiko;Hokfelt, Tomas;Gebhart, G. F.
关键词：
谷氨酸是神经系统中的主要兴奋性神经递质，包括初级传入神经元。然而，到目前为止，自主神经的谷氨酸能表型还没有被描述。为此，我们采用免疫组织化学和原位杂交的方法，研究了囊泡型谷氨酸转运体(VGLUT)在幼龄BALB/C小鼠腰交感链(LSC)和盆神经节(MPG)以及盆神经切断(PNA)后的表达。同时检测激活转录因子-3(ATF-3)、酪氨酸羟化酶(TH)、囊泡乙酰胆碱转运体(VAChT)和降钙素相关肽的共定位。包括假PNA、坐骨神经切断(SNA)或幼稚小鼠。在幼稚小鼠，VGLUT2样免疫反应(LI)仅见于LSC和MPG的纤维和静脉曲张，未见ATF-3免疫反应(IR)神经元。相反，PNA诱导LSC神经元亚群的VGLUT2蛋白和转录以及ATF-3-LI的表达上调。有趣的是，VGLUT2-IR LSC神经元共表达ATF-3，并且通常缺乏去甲肾上腺素能标记TH。SNA仅增加散在的LSC神经元中VGLUT2蛋白和转录水平。PNA和SNA均不能上调MPG神经元的VGLUT2。我们还发现，在非PNA的MPG中，VGLUT2或乙酰胆碱能标志Vacht的神经节周围有VGLUT2或Vacht的免疫反应，通常在TH-IR神经元周围。VGLUT1-LI仅限于MPGs中的一些曲张，在LSC中不存在，并且基本不受PNA或SNA的影响。即使在PNA或SNA后，LSC中也没有VGLUT1或VGLUT3mRNAs的表达，这一点得到了证实。综上所述，切断内脏神经和非内脏神经可导致一些LSC神经元的谷氨酸能表型。此外，我们还展示了以前未描述的MPG神经元周围谷氨酸能篮子。
Glutamate is the main excitatory neurotransmitter in the nervous system, including in primary afferent neurons. However, to date a glutamatergic phenotype of autonomic neurons has not been described. Therefore, we explored the expression of vesicular glutamate transporters (VGLUTs) type 1, 2 and 3 in lumbar sympathetic chain (LSC) and major pelvic ganglion (MPG) of naïve BALB/C mice, as well as after pelvic nerve axotomy (PNA), using immunohistochemistry and in situ hybridization. Colocalization with activating transcription factor-3 (ATF-3), tyrosine hydroxylase (TH), vesicular acetylcholine transporter (VAChT) and calcitonin generelated peptide was also examined. Sham-PNA, sciatic nerve axotomy (SNA) or naïve mice were included. In naïve mice, VGLUT2-like immunoreactivity (LI) was only detected in fibers and varicosities in LSC and MPG; no ATF-3-immunoreactive (IR) neurons were visible. In contrast, PNA induced upregulation of VGLUT2 protein and transcript, as well as of ATF-3-LI in subpopulations of LSC neurons. Interestingly, VGLUT2-IR LSC neurons coexpressed ATF-3, and often lacked the noradrenergic marker TH. SNA only increased VGLUT2 protein and transcript in scattered LSC neurons. Neither PNA nor SNA upregulated VGLUT2 in MPG neurons. We also found perineuronal baskets immunoreactive either for VGLUT2 or the acetylcholinergic marker VAChT in non-PNA MPGs, usually around TH-IR neurons. VGLUT1-LI was restricted to some varicosities in MPGs, was absent in LSCs, and remained largely unaffected by PNA or SNA. This was confirmed by the lack of expression of VGLUT1 or VGLUT3 mRNAs in LSCs, even after PNA or SNA. Taken together, axotomy of visceral and non-visceral nerves results in a glutamatergic phenotype of some LSC neurons. In addition, we show previously non-described MPG perineuronal glutamatergic baskets.
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影响因子：
3.3
作者：
Dickerson, J. W.;Hemmerle, A. M.;Numan, S.;Lundgren, K. H.;Seroogy, K. B.
通讯作者：
Seroogy, K. B.
影响因子：
2.9
作者：
Brumovsky, PR;Bergman, E;Villar, MJ
通讯作者：
Villar, MJ
影响因子：
7.4
作者：
BENNETT, GJ;XIE, YK
通讯作者：
XIE, YK
影响因子：
7.4
作者：
Coggeshall, RE;Carlton, SM
通讯作者：
Carlton, SM
影响因子：
2.4
作者：
Costigan M;Befort K;Karchewski L;Griffin RS;D'Urso D;Allchorne A;Sitarski J;Mannion JW;Pratt RE;Woolf CJ
通讯作者：
Woolf CJ