Using Pox-Neuro (Poxn) Mutants in Drosophila Gustation Research: A Double-Edged Sword.
Using Pox-Neuro (Poxn) Mutants in Drosophila Gustation Research: A Double-Edged Sword.
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DOI：
10.3389/fncel.2018.00382
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发表时间：
2018
影响因子：
5.3
通讯作者：
Dahanukar A
中科院分区：
文献类型：
--
作者：
Chen YD;Park SJ;Ja WW;Dahanukar A
在果蝇中，Pox-neuro（Poxn）是Paired box（Pax）基因家族的成员，其编码具有特征性配对DNA结合结构域的转录因子。在胚胎发育期间，Poxn在多神经支配的外部感觉（p-es）器官的感觉器官前体（SOP）细胞中表达，并且对于指定p-es器官身份（化学感觉）而不是单神经支配的外部感觉（m-es）器官（机械感觉）是重要的。在Poxn突变体中，化学感受刚毛转化为机械感受刚毛。因此，这些突变体通常被认为是完全味觉盲的，研究人员利用它们来研究以味觉独立方式起作用的生理和行为功能。然而，最近的研究表明，只有外部味觉刚毛转化为POXn突变体，而所有内部咽味觉神经元保持完整，引起了人们对使用POXn突变体作为味觉盲苍蝇的实验结果的解释的关注。在这篇综述中，我们总结了Poxn突变体在推进我们的味觉丰富的基因和摄食行为的知识的价值，并鼓励重新审视一些来自这些突变体的味觉无关的营养传感机制的结论。最后，我们强调，Poxn突变苍蝇仍然是一个有价值的工具，用于探测相对欠研究的咽部味觉神经元在感知膳食特性和调节摄食行为的功能。
In Drosophila, Pox-neuro (Poxn) is a member of the Paired box (Pax) gene family that encodes transcription factors with characteristic paired DNA-binding domains. During embryonic development, Poxn is expressed in sensory organ precursor (SOP) cells of poly-innervated external sensory (p-es) organs and is important for specifying p-es organ identity (chemosensory) as opposed to mono-innervated external sensory (m-es) organs (mechanosensory). In Poxn mutants, there is a transformation of chemosensory bristles into mechanosensory bristles. As a result, these mutants have often been considered to be entirely taste-blind, and researchers have used them in this capacity to investigate physiological and behavioral functions that act in a taste-independent manner. However, recent studies show that only external taste bristles are transformed in Poxn mutants whereas all internal pharyngeal taste neurons remain intact, raising concerns about interpretations of experimental results using Poxn mutants as taste-blind flies. In this review, we summarize the value of Poxn mutants in advancing our knowledge of taste-enriched genes and feeding behaviors, and encourage revisiting some of the conclusions about taste-independent nutrient-sensing mechanisms derived from these mutants. Lastly, we highlight that Poxn mutant flies remain a valuable tool for probing the function of the relatively understudied pharyngeal taste neurons in sensing meal properties and regulating feeding behaviors.
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影响因子：
16.6
作者：
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通讯作者：
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