Respiratory mechanics do not always mirror pulmonary histological changes in emphysema.
Respiratory mechanics do not always mirror pulmonary histological changes in emphysema.
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DOI：
10.1590/s1807-59322011001000020
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Lopes FD
中科院分区：
文献类型：
--
作者：
Anciães AM;Olivo CR;Prado CM;Kagohara KH;Pinto Tda S;Moriya HT;Mauad T;Martins Mde A;Lopes FD
验证肺气肿发生过程中功能和形态学参数的一致性。BALB/c小鼠接受木瓜蛋白酶或盐水溶液的滴鼻，并在1、3、15、28和40天后进行研究。功能参数，如气道阻力，组织阻尼，和组织弹性，进行了分析。为了评估这种疾病的结构变化和可能的机制，我们测量了平均线性截距，弹性和胶原纤维的体积比例，巨噬细胞的数量，表达金属蛋白酶12和8-异前列腺素的细胞数量。：我们仅在第28天观察到组织弹性和组织阻尼降低，平均线性截距随之增加，表明存在肺气肿。然而，只有平均线性截距值保持增加，直到第40天。胶原纤维的体积比例从第15天到第40天增加，而弹性纤维的体积比例仅在第40天增加。巨噬细胞数量从第1天开始增加。基质金属蛋白酶12的表达从第3天开始逐渐增加，直至第40天。然而，8-异前列烷的表达仅在第1天和第3天增加。在这项研究中，形态参数被发现是更可靠的检测肺气肿的存在比呼吸力学测量的功能参数。进一步的研究是必要的，以了解如何在肺实质中观察到的细胞外基质重塑可能参与这一过程。
: To verify the accordance of functional and morphometric parameters during the development of emphysema. : BALB/c mice received a nasal drop of either papain or saline solution and were studied after 1, 3, 15, 28, and 40 days. Functional parameters, such as airway resistance, tissue damping, and tissue elastance, were analyzed. To evaluate the structural changes and possible mechanisms involved in this disease, we measured the mean linear intercept, the volume proportions of elastic and collagen fibers, the number of macrophages, the numbers of cells expressing metalloprotease 12 and 8-isoprostane in lung parenchyma. : We only observed decreases in tissue elastance and tissue damping on the 28th day, with a concomitant increase in the mean linear intercept, indicating the presence of emphysema. However, only the mean linear intercept values remained increased until the 40th day. The volume proportion of collagen fibers was increased from the 15th day to the 40th day, whereas the volume proportion of elastic fibers was only increased on the 40th day. The number of macrophages increased beginning on the 1st day. The expression of metalloproteinase 12 was increased from the 3rd day until the 40th day. However, 8-isoprostane expression was only increased on the 1st and 3rd days. : In this study, morphometric parameters were found to be more reliable for detecting the presence of emphysema than the functional parameters measured by respiratory mechanics. Further investigations are necessary to understand how the extracellular matrix remodeling observed in the lung parenchyma could be involved in this process.
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影响因子：
3.3
作者：
Ito, S;Ingenito, EP;Suki, B
通讯作者：
Suki, B
DOI：
10.1164/ajrccm.156.1.9612018
发表时间：
1997-07-01
影响因子：
24.7
作者：
Finlay, GA;ODriscoll, LR;OConnor, CM
通讯作者：
OConnor, CM
影响因子：
2.1
作者：
March, TH;Green, FHY;Nikula, KJ
通讯作者：
Nikula, KJ
影响因子：
8.3
作者：
Lopes, Fernanda D. T. Q. S.;Pinto, Tatiana S.;Martins, Milton A.
通讯作者：
Martins, Milton A.
影响因子：
2
作者：
Pastor, Luis Miguel;Sanchez-Gascon, F.;Canteras, M.
通讯作者：
Canteras, M.