Differential uptake of FDG and DG during post-ischaemic reperfusion in the isolated, perfused rat heart
Differential uptake of FDG and DG during post-ischaemic reperfusion in the isolated, perfused rat heart
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离体灌注大鼠心脏缺血再灌注期间 FDG 和 DG 的摄取差异
DOI：
10.1007/s002590050595
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
P. Marsden
中科院分区：
文献类型：
--
作者：
P. Garlick;R. Medina;R. Southworth;P. Marsden
抽象的。氟-18 2-氟-2-脱氧葡萄糖（FDG）和2-脱氧葡萄糖（DG）被广泛用作心肌中葡萄糖摄取的示踪剂。尽管一致认为这两种类似物在对照条件下的行为与葡萄糖相似，但越来越多的证据表明，某些干预措施（如胰岛素刺激或缺血/再灌注）会导致其行为发生差异性变化。在我们最近开发的PET和NMR双采集（PANDA）系统中添加了双表面线圈核磁共振（NMR）探头和双灌注插管，使我们能够同时从每个独立灌注的心脏冠状动脉床收集PET（FDG）图像和磷-31 NMR（2-脱氧葡萄糖-6-磷酸）光谱。我们已经使用这种技术来研究区域缺血/再灌注对FDG和DG摄取的影响，在离体灌注大鼠心脏。在对照灌注期间，两个冠状动脉床的FDG摄取几乎相同。当一个冠状动脉床缺血时，FDG摄取在该侧停止，但在对照侧继续。再灌注未能恢复FDG摄取。与此相反，核磁共振谱显示，在再灌注期间，DG的摄取和磷酸化没有两个冠状动脉床之间的差异。结果表明，局部心肌缺血/再灌注对离体灌流大鼠心脏摄取FDG和DG有不同的影响。
Abstract. Fluorine-18 2-fluoro-2-deoxyglucose (FDG) and 2-deoxyglucose (DG) are widely used as tracers of glucose uptake in the myocardium. Although there is agreement that the two analogues behave similarly to glucose under control conditions, there is growing evidence that some interventions (e.g. insulin stimulation or ischaemia/reperfusion) cause differential changes in their behaviour. The addition of a two-surface coil nuclear magnetic resonance (NMR) probe and a dual-perfusion cannula to our recently developed PET and NMR dual-acquisition (PANDA) system allows us to collect PET (FDG) images and phosphorus-31 NMR (2-deoxyglucose-6-phosphate) spectra simultaneously from each independently perfused coronary bed of the heart. We have used this technique to study the effect of regional ischaemia/reperfusion on FDG and DG uptake in the isolated, perfused rat heart. During control perfusion, FDG uptake was almost identical in both coronary beds. When one coronary bed was made ischaemic, FDG uptake ceased on that side but continued on the control side. Reperfusion failed to restore FDG uptake. In contrast, NMR spectra showed that, during reperfusion, the uptake and phosphorylation of DG did not differ between the two coronary beds. The results thus demonstrate that regional myocardial ischaemia/reperfusion has different effects on the uptake of FDG and DG in the isolated, perfused rat heart.
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DOI：
10.1016/0167-4889(82)90034-9
发表时间：
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期刊：
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