Raman spectroscopy and regenerative medicine: a review.
Raman spectroscopy and regenerative medicine: a review.
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DOI：
10.1038/s41536-017-0014-3
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发表时间：
2017
影响因子：
7.2
通讯作者：
Campbell CJ
中科院分区：
文献类型：
--
作者：
Ember KJI;Hoeve MA;McAughtrie SL;Bergholt MS;Dwyer BJ;Stevens MM;Faulds K;Forbes SJ;Campbell CJ
再生医学的领域涵盖了生物医学的广泛领域，从实验室中的单细胞培养到人类全器官移植。为了确保研究从实验室转移到床边，我们能够在生化水平上评估细胞，组织，器官和患者的再生过程至关重要。再生依赖于大量的生物因素，这些因素可以使用常规的生物分析技术进行干扰。一种通用的，非侵入性的，非破坏性的生化分析技术将是非常宝贵的再生研究，和拉曼光谱是一个潜在的解决方案。拉曼光谱是一种通过光的非弹性散射获得化学数据的分析方法。自20世纪20年代发现拉曼散射以来，物理学家和化学家一直使用拉曼散射来研究各种液体和固体材料的化学成分。然而，只有在过去的二十年中，这种形式的光谱学才被用于生物医学研究。与再生医学特别相关的是最近的研究，说明了其在细胞、组织活检和患者中识别和区分健康和疾病状态的能力。这篇综述将简要概述拉曼光谱及其变体背后的原理，描述其应用于生物医学的关键例子，并考虑再生医学领域，将受益于这种非侵入性的生物分析工具。
The field of regenerative medicine spans a wide area of the biomedical landscape—from single cell culture in laboratories to human whole-organ transplantation. To ensure that research is transferrable from bench to bedside, it is critical that we are able to assess regenerative processes in cells, tissues, organs and patients at a biochemical level. Regeneration relies on a large number of biological factors, which can be perturbed using conventional bioanalytical techniques. A versatile, non-invasive, non-destructive technique for biochemical analysis would be invaluable for the study of regeneration; and Raman spectroscopy is a potential solution. Raman spectroscopy is an analytical method by which chemical data are obtained through the inelastic scattering of light. Since its discovery in the 1920s, physicists and chemists have used Raman scattering to investigate the chemical composition of a vast range of both liquid and solid materials. However, only in the last two decades has this form of spectroscopy been employed in biomedical research. Particularly relevant to regenerative medicine are recent studies illustrating its ability to characterise and discriminate between healthy and disease states in cells, tissue biopsies and in patients. This review will briefly outline the principles behind Raman spectroscopy and its variants, describe key examples of its applications to biomedicine, and consider areas of regenerative medicine that would benefit from this non-invasive bioanalytical tool.
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影响因子：
18.2
作者：
Bergholt, Mads S.;St-Pierre, Jean-Philippe;Offeddu, Giovanni S.;Parmar, Paresh A.;Albro, Michael B.;Puetzer, Jennifer L.;Oyen, Michelle L.;Stevens, Molly M.
通讯作者：
Stevens, Molly M.
影响因子：
3.4
作者：
Chan, JW;Taylor, DS;Huser, T
通讯作者：
Huser, T
影响因子：
7.4
作者：
Driskell, Jeremy D.;Uhlenkamp, Jill M.;Porter, Marc D.
通讯作者：
Porter, Marc D.
影响因子：
7.4
作者：
Bergholt, Mads Sylvest;Zheng, Wei;Huang, Zhiwei
通讯作者：
Huang, Zhiwei
影响因子：
2.5
作者：
Downes, Andrew;Mouras, Rabah;Bagnaninchi, Pierre;Elfick, Alistair
通讯作者：
Elfick, Alistair