Raman spectroscopy and CARS microscopy of stem cells and their derivatives.
Raman spectroscopy and CARS microscopy of stem cells and their derivatives.
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DOI：
10.1002/jrs.2975
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发表时间：
2011-10
影响因子：
2.5
通讯作者：
Elfick, Alistair
中科院分区：
文献类型：
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作者：
Downes, Andrew;Mouras, Rabah;Bagnaninchi, Pierre;Elfick, Alistair
干细胞的特性对再生医学至关重要。在治疗前未能将所有干细胞从分化细胞中分离出来可能会导致畸胎瘤-多种细胞类型的肿瘤。通常，表征是以破坏性的方式用荧光测定法进行的。一种真正的非侵入性表征方法将是基于干细胞疗法的重大突破。拉曼光谱显示，当干细胞分化为其他细胞类型时，DNA和RNA水平下降，我们将其与细胞核和细胞质相对大小的变化联系起来。我们还使用拉曼光谱来研究早期胚胎或胚泡内的生物化学，这与胚胎干细胞集落有很大不同。从干细胞分化的某些细胞类型可以通过汽车显微镜直接对生物化学进行成像来识别;所提供的例子是羟基磷灰石-骨的前体和脂肪细胞中的脂质。
The characterisation of stem cells is of vital importance to regenerative medicine. Failure to separate out all stem cells from differentiated cells before therapies can result in teratomas – tumours of multiple cell types. Typically, characterisation is performed in a destructive manner with fluorescent assays. A truly non-invasive method of characterisation would be a major breakthrough in stem cell-based therapies. Raman spectroscopy has revealed that DNA and RNA levels drop when a stem cell differentiates into other cell types, which we link to a change in the relative sizes of the nucleus and cytoplasm. We also used Raman spectroscopy to investigate the biochemistry within an early embryo, or blastocyst, which differs greatly from colonies of embryonic stem cells. Certain cell types that differentiate from stem cells can be identified by directly imaging the biochemistry with CARS microscopy; examples presented are hydroxyapatite – a precursor to bone, and lipids in adipocytes.
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影响因子：
46.9
作者：
Blum, Barak;Bar-Nur, Ori;Benvenisty, Nissim
通讯作者：
Benvenisty, Nissim
影响因子：
6.2
作者：
Gourion-Arsiquaud, Samuel;Burket, Jayme C.;Boskey, Adele L.
通讯作者：
Boskey, Adele L.
影响因子：
--
作者：
Downes A;Mouras R;Elfick A
通讯作者：
Elfick A
影响因子：
3.6
作者：
Kee, TW;Cicerone, MT
通讯作者：
Cicerone, MT
影响因子：
--
作者：
MAKER, PD;TERHUNE, RW
通讯作者：
TERHUNE, RW