Human dendritic cells (DCs) are derived from distinct circulating precursors that are precommitted to become CD1c+ or CD141+ DCs.
Human dendritic cells (DCs) are derived from distinct circulating precursors that are precommitted to become CD1c+ or CD141+ DCs.
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DOI：
10.1084/jem.20161135
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发表时间：
2016-12-12
期刊：
影响因子：
--
通讯作者：
Nussenzweig MC
中科院分区：
文献类型：
--
作者：
Breton G;Zheng S;Valieris R;Tojal da Silva I;Satija R;Nussenzweig MC
Breton等人。鉴定CD172a是区分人类CD1c+亚群(CD172a+前CDC)或CD141+亚群(CD172a−前CDC)的谱系标志物。在人类中，传统的树突状细胞(CDCs)以CD1c和CD141的表达为特征的两种独特的群体存在。CDC起源于越来越有限但定义明确的骨髓前体细胞，其中包括分化为前CDC的常见DC前体，前CDC是CDC的直接前体。在这项研究中，我们发现人类中的前CDC是异质性的，由两个不同的前体群体组成，它们被预先承诺为CD1c+或CD141+CDC。根据CD172a的差异表达可以区分这两组谱系启动的前体细胞。这两种前CDC亚群都出现在成人骨髓中，并可在脐带血和成人外周血中发现。基因表达分析表明，CD172a+和CD172a−前CDC代表发育不连续的群体，它们差异表达谱系限制性转录因子。Flt3L注射的临床试验表明，该细胞因子可增加人外周血中CD172a−和CD172a+前CDC的数量。
Breton et al. identify CD172a as a lineage marker that distinguishes human cDC precursor (pre-cDC) subpopulations committed to the CD1c+ lineage (CD172a+ pre-cDCs) or CD141+ lineage (CD172a− pre-cDCs). In humans, conventional dendritic cells (cDCs) exist as two unique populations characterized by expression of CD1c and CD141. cDCs arise from increasingly restricted but well-defined bone marrow progenitors that include the common DC progenitor that differentiates into the pre-cDC, which is the direct precursor of cDCs. In this study, we show that pre-cDCs in humans are heterogeneous, consisting of two distinct populations of precursors that are precommitted to become either CD1c+ or CD141+ cDCs. The two groups of lineage-primed precursors can be distinguished based on differential expression of CD172a. Both subpopulations of pre-cDCs arise in the adult bone marrow and can be found in cord blood and adult peripheral blood. Gene expression analysis revealed that CD172a+ and CD172a− pre-cDCs represent developmentally discrete populations that differentially express lineage-restricted transcription factors. A clinical trial of Flt3L injection revealed that this cytokine increases the number of both CD172a− and CD172a+ pre-cDCs in human peripheral blood.
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影响因子：
64.8
作者：
Shalek, Alex K.;Satija, Rahul;Shuga, Joe;Trombetta, John J.;Gennert, Dave;Lu, Diana;Chen, Peilin;Gertner, Rona S.;Gaublomme, Jellert T.;Yosef, Nir;Schwartz, Schraga;Fowler, Brian;Weaver, Suzanne;Wang, Jing;Wang, Xiaohui;Ding, Ruihua;Raychowdhury, Raktima;Friedman, Nir;Hacohen, Nir;Park, Hongkun;May, Andrew P.;Regev, Aviv
通讯作者：
Regev, Aviv
DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
30.5
作者：
Grajales-Reyes GE;Iwata A;Albring J;Wu X;Tussiwand R;Kc W;Kretzer NM;Briseño CG;Durai V;Bagadia P;Haldar M;Schönheit J;Rosenbauer F;Murphy TL;Murphy KM
通讯作者：
Murphy KM
影响因子：
8.7
作者：
Manicassamy S;Pulendran B
通讯作者：
Pulendran B
DOI：
10.1084/jem.20141441
发表时间：
2015-03-09
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Breton G;Lee J;Zhou YJ;Schreiber JJ;Keler T;Puhr S;Anandasabapathy N;Schlesinger S;Caskey M;Liu K;Nussenzweig MC
通讯作者：
Nussenzweig MC