Inhibition of Epithelial-Mesenchymal Transition Maintains Stemness in Human Amniotic Epithelial Cells.
Inhibition of Epithelial-Mesenchymal Transition Maintains Stemness in Human Amniotic Epithelial Cells.
复制标题
DOI：
10.1007/s12015-022-10420-1
复制
发表时间：
2022-12
影响因子：
4.8
通讯作者：
Miki, Toshio
中科院分区：
文献类型：
--
作者：
Takano, Chika;Horie, Masafumi;Taiko, Isamu;Quang Duy Trinh;Kanemaru, Kazunori;Komine-Aizawa, Shihoko;Hayakawa, Satoshi;Miki, Toshio
关键词：
人羊膜上皮细胞（hAEC）是一种胎盘干细胞，表达干细胞标记基因，并且能够在适当的培养条件下分化成所有三个胚层。已知hAEC经历TGF-β依赖性上皮-间充质转化（EMT）;然而，EMT对hAEC的干性或分化的影响尚未确定。在这里，我们首先证实了hAEC在开始原代培养后立即经历EMT。使用RNA-seq的综合转录组分析显示，抑制TGF-β依赖性EMT维持了hAEC中干细胞相关基因的表达，包括NANOG和POU 5 F1。此外，干性的维持并不影响hAEC的非致瘤性特征。我们首次发现TGF-β依赖性EMT对hAEC的干细胞性产生负面影响，为胎盘干细胞的细胞过程提供了新的见解。在线版本包含补充材料，可通过10.1007/s12015-022-10420-1获得。
Human amniotic epithelial cells (hAECs), which are a type of placental stem cell, express stem cell marker genes and are capable of differentiating into all three germ layers under appropriate culture conditions. hAECs are known to undergo TGF-β-dependent epithelial-mesenchymal transition (EMT); however, the impact of EMT on the stemness or differentiation of hAECs has not yet been determined. Here, we first confirmed that hAECs undergo EMT immediately after starting primary culture. Comprehensive transcriptome analysis using RNA-seq revealed that inhibition of TGF-β-dependent EMT maintained the expression of stemness-related genes, including NANOG and POU5F1, in hAECs. Moreover, the maintenance of stemness did not affect the nontumorigenic characteristics of hAECs. We showed for the first time that TGF-β-dependent EMT negatively affected the stemness of hAECs, providing novel insight into cellular processes of placental stem cells. The online version contains supplementary material available at 10.1007/s12015-022-10420-1.
登录
查看更多内容
影响因子：
--
作者：
Miki, Toshio;Marongiu, Fabio;C Strom, Stephen
通讯作者：
C Strom, Stephen
影响因子：
7.5
作者：
Miki T
通讯作者：
Miki T
影响因子：
1.6
作者：
Miki, Toshio;Grubbs, Brendan
通讯作者：
Grubbs, Brendan
影响因子：
--
作者：
Soneson C;Love MI;Robinson MD
通讯作者：
Robinson MD
影响因子：
3.7
作者：
Costa-Silva J;Domingues D;Lopes FM
通讯作者：
Lopes FM