Characterization of phytohormone and transcriptome profiles during protocorm-like bodies development of Paphiopedilum.
Characterization of phytohormone and transcriptome profiles during protocorm-like bodies development of Paphiopedilum.
复制标题
兜兰类原球茎发育过程中植物激素和转录组谱的表征
DOI：
10.1186/s12864-021-08087-y
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发表时间：
2021-11-08
期刊：
影响因子：
4.4
通讯作者：
Fang L
中科院分区：
文献类型：
--
作者：
Guo B;Zeng S;Yin Y;Li L;Ma G;Wu K;Fang L
背景
兜兰，俗称拖鞋兰，是兰科的一个重要属，具有突出的园艺价值。与传统的分蘖和离体芽增殖方法相比，类原球茎诱导和再生是兜兰属植物快速繁殖的有效方法。不同种类、不同杂交种和不同品种的原球茎诱导效率不同，导致只有少数兜兰属植物可以通过原球茎进行大规模繁殖。到目前为止，对兜兰类植物原球茎发生和维持的机制知之甚少。
结果
建立了以兜兰（P.SCBG Prince × P.SCBG Miracle）的原球茎为外植体诱导原球茎发生原球茎的方法。四个关键PLB发育阶段的形态特征表明，在每个PLB内观察到显着的极性和细胞大小梯度。评价内源性激素水平。吲哚乙酸（IAA）和茉莉酸（JA）含量的增加伴随着原球茎的分化，表明生长素和JA含量与原球茎的发育相关。赤霉酸（GA）含量下降到很低的水平，表明GA失活可能是茎尖分生组织（SAM）发育所必需的。
通过对辉黄90原球茎4个不同发育阶段转录组的比较，探讨了原球茎发育的关键基因。在三个成对比较（A与B、B与C、C与D）中差异表达基因（DEG）的数目分别为1455、349和3529。KEGG富集分析表明，DEGs参与次生代谢物代谢和光合作用。详细讨论了与激素代谢和信号传导、体细胞胚胎发生、芽发育和光合作用有关的DEG。
结论
本研究首次利用转录组测序技术对兜兰类原球茎发育进行了研究，为了解兜兰类原球茎的发育机制提供了有用的信息。
Background
Paphiopedilum, commonly known as slipper orchid, is an important genus of orchid family with prominent horticultural value. Compared with conventional methods such as tillers and in vitro shoots multiplication, induction and regeneration of protocorm-like bodies (PLBs) is an effective micropropagation method in Paphiopedilum. The PLB initiation efficiency varies among species, hybrids and varieties, which leads to only a few Paphiopedilum species can be large-scale propagated through PLBs. So far, little is known about the mechanisms behind the initiation and maintenance of PLB in Paphiopedilum.
Results
A protocol to induce PLB development from seed-derived protocorms of Paphiopedilum SCBG Huihuang90 (P. SCBG Prince × P. SCBG Miracle) was established. The morphological characterization of four key PLB developmental stages showed that significant polarity and cell size gradients were observed within each PLB. The endogenous hormone level was evaluated. The increase in the levels of indoleacetic acid (IAA) and jasmonic acid (JA) accompanying the PLBs differentiation, suggesting auxin and JA levels were correlated with PLB development. Gibberellic acid (GA) decreased to a very low level, indicated that GA inactivation may be necessary for shoot apical meristem (SAM) development.
Comparative transcriptomic profiles of four different developmental stages of P. SCBG Huihuang90 PLBs explore key genes involved in PLB development. The numbers of differentially expressed genes (DEGs) in three pairwise comparisons (A vs B, B vs C, C vs D) were 1455, 349, and 3529, respectively. KEGG enrichment analysis revealed that DEGs were implicated in secondary metabolite metabolism and photosynthesis. DEGs related to hormone metabolism and signaling, somatic embryogenesis, shoot development and photosynthesis were discussed in detail.
Conclusion
This study is the first report on PLB development in Paphiopedilum using transcriptome sequencing, which provides useful information to understand the mechanisms of PLB development.
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