Weak interaction-alleviated toxicity of aromatic compounds in EPS matrices: Quantifying the noncovalent bonding-to-EPS ecoservice chain.
Weak interaction-alleviated toxicity of aromatic compounds in EPS matrices: Quantifying the noncovalent bonding-to-EPS ecoservice chain.
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EPS 基质中芳香族化合物的弱相互作用减轻毒性：量化 EPS 生态服务链的非共价键合。
DOI：
10.1016/j.jhazmat.2021.125824
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发表时间：
2021-04
影响因子：
13.6
通讯作者：
Peilin Liu
中科院分区：
文献类型：
--
作者：
Kai Chen;Qian Wang;Yaojia Fu;Fuxing Kang;Xiaoxiao Yu;Peilin Liu
胞外聚合物（EPS）是一种广泛存在于全球的碳库，参与有机化学物质的地球化学过程。除了由EPS施加的化学水解和化学品的氧化还原，与分散的EPS的弱非共价相互作用控制许多有机化合物的毒性。然而，对这一问题缺乏深入的研究。本研究利用天然生物膜量化了从键合到解毒的一系列环节，以探索脆弱的非共价键合对芳香族化合物生态毒性的控制行为。这种结合使细胞对间苯二胺、2-萘酚和菲的吸收能力降低5.3- 53.6%，使微生物多样性指数提高122.2- 279.5%。在本文中，EPS中的60 kDa伴侣蛋白作为非共价相互作用的最重要贡献者（前20种蛋白质的16.4%）。EPS中的亲水性羧基分别与间苯二胺和2-萘酚的羟基和氨基通过氢键结合。亚甲基和羧基分别通过CH···π和H-键与疏水菲联合收割机结合。因此，建立了一个量化链，弱相互作用线性控制的生态毒性的芳香族化合物通过上述抑制细胞吸收的芳香族化合物（R2= 0.82）。考虑到无处不在的EPS和流行的芳香族化合物，我们的研究结果揭示了一个以前未被注意的现象，其中看似脆弱的非共价键合深刻地揭示了芳香族化合物在地球表面系统中的生态毒性。
Extracellular polymeric substances (EPSs) constitute a largely global carbon pool that could participate in geochemical process of organic chemicals. Besides the chemical hydrolysis and redox of chemicals exerted by the EPS, weakly noncovalent interactions with dispersive EPS control the toxicity of numerous organic compounds. Nevertheless, there has been a lack of in-depth research on this issue. This work quantified a chain of links from bonding to detoxification using natural biofilms to explore the control behavior of fragile noncovalent bonding to the ecotoxicity of aromatic compounds. Such bonding decreases cell absorbability ofm-phenylenediamine, 2-naphthol, and phenanthrene by 5.3–53.6%, resultantly increasing the indices of microbial diversity by 122.2–279.5%. Herein, the 60 kDa chaperonin in EPS acts as the most important contributor (16.4% of the top 20 proteins) to noncovalent interactions. Hydrophilic carboxyl groups in EPS bind with hydroxyl and amino groups ofm-phenylenediamine and 2-naphthol via H-bonds, respectively. Methylene and carboxyl groups combine with hydrophobic phenanthrene via CH···π and H-bonding, respectively. A quantified chain was consequently established that weak interaction linearly controls ecotoxicity of aromatic compounds via the above suppressive cell absorbability of aromatic compounds (R2= 0.82). Considering ubiquitous EPS and prevailing aromatic compounds, our findings revealed a previously unnoticed phenomenon in which seemingly fragile noncovalent bonding profoundly alleviates the ecotoxicity of aromatic compounds in Earth’s surface system.
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