Is TrpM5 a reliable marker for chemosensory cells? Multiple types of microvillous cells in the main olfactory epithelium of mice.
Is TrpM5 a reliable marker for chemosensory cells? Multiple types of microvillous cells in the main olfactory epithelium of mice.
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DOI：
10.1186/1471-2202-9-115
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发表时间：
2008-12-04
期刊：
影响因子：
2.4
通讯作者：
Finger, Thomas E.
中科院分区：
文献类型：
--
作者：
Hansen, Anne;Finger, Thomas E.
过去，纤毛受体神经元、基底细胞和支持细胞被认为是主嗅上皮的主要组成部分。一些研究报道了微绒毛细胞的存在，但其功能尚不清楚。最近的一项研究表明，在哺乳动物的主要嗅觉上皮细胞中表达瞬时受体电位通道TrpM5，这些细胞具有化学感觉功能，而TrpM5是哺乳动物化学感觉器官的内在信号成分。我们询问嗅觉上皮中的trpm5阳性细胞是否为微绒毛细胞，它们是否属于化学感觉系统，即嗅觉神经元或三叉神经支配的孤立化学感觉细胞。我们在光镜和电镜下研究了小鼠的主要嗅上皮，并描述了微绒毛细胞的几个亚群。微绒毛细胞的超微结构显示出至少三种形态不同的类型，其中两种表达TrpM5通道。这些细胞都没有投射到嗅球的轴突。用大量细胞标记物进行的测试表明，trpm5阳性细胞不是感觉细胞，因为它们既不表达神经元标记物，也不与三叉神经纤维接触。我们得出结论，TrpM5不是化学感觉细胞的可靠标记。嗅觉上皮的trpm5阳性细胞呈微绒毛状，虽然不是感觉器官的一部分，但可能具有化学反应性。然而，这些微绒毛细胞的活性可能影响嗅觉系统局部元件的功能。
In the past, ciliated receptor neurons, basal cells, and supporting cells were considered the principal components of the main olfactory epithelium. Several studies reported the presence of microvillous cells but their function is unknown. A recent report showed cells in the main olfactory epithelium that express the transient receptor potential channel TrpM5 claiming that these cells are chemosensory and that TrpM5 is an intrinsic signaling component of mammalian chemosensory organs. We asked whether the TrpM5-positive cells in the olfactory epithelium are microvillous and whether they belong to a chemosensory system, i.e. are olfactory neurons or trigeminally-innervated solitary chemosensory cells. We investigated the main olfactory epithelium of mice at the light and electron microscopic level and describe several subpopulations of microvillous cells. The ultrastructure of the microvillous cells reveals at least three morphologically different types two of which express the TrpM5 channel. None of these cells have an axon that projects to the olfactory bulb. Tests with a large panel of cell markers indicate that the TrpM5-positive cells are not sensory since they express neither neuronal markers nor are contacted by trigeminal nerve fibers. We conclude that TrpM5 is not a reliable marker for chemosensory cells. The TrpM5-positive cells of the olfactory epithelium are microvillous and may be chemoresponsive albeit not part of the sensory apparatus. Activity of these microvillous cells may however influence functionality of local elements of the olfactory system.
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DOI：
10.1073/pnas.94.7.3388
发表时间：
1997-04-01
影响因子：
11.1
作者：
Juilfs, DM;Fulle, HJ;Beavo, JA
通讯作者：
Beavo, JA
影响因子：
2.9
作者：
MILLER, ML;ANDRINGA, A;HASTINGS, L
通讯作者：
HASTINGS, L
DOI：
10.1159/000276268
发表时间：
1992-03-01
期刊：
ORL-JOURNAL FOR OTO-RHINO-LARYNGOLOGY AND ITS RELATED SPECIALTIES
影响因子：
--
作者：
YAMAGISHI, M;NAKAMURA, H;KUWANO, R
通讯作者：
KUWANO, R
影响因子：
2.5
作者：
MULLER, JF;MARC, RE
通讯作者：
MARC, RE
DOI：
10.1016/s0022-5320(67)80137-0
发表时间：
1967-01-01
期刊：
JOURNAL OF ULTRASTRUCTURE RESEARCH
影响因子：
--
作者：
OKANO, M;WEBER, AF;FROMMES, SP
通讯作者：
FROMMES, SP