A shut-down in expression of an insulin-like peptide, ILP-1, halts ovarian maturation during the overwintering diapause of the mosquito Culex pipiens.
A shut-down in expression of an insulin-like peptide, ILP-1, halts ovarian maturation during the overwintering diapause of the mosquito Culex pipiens.
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DOI：
10.1111/j.1365-2583.2009.00872.x
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发表时间：
2009-06
影响因子：
2.6
通讯作者：
Denlinger DL
中科院分区：
文献类型：
--
作者：
Sim C;Denlinger DL
短日照长度被用于对蚊库蚊（Culex pipiens）的成虫滞育编程。停止卵巢成熟的下游内分泌事件是保幼激素（JH）产生的关闭，最近的证据表明，胰岛素信号通路可能是执行这种发育停滞的关键上游参与者。在C. pipiens，我们报告说，转录水平的胰岛素样肽-1和-5滞育女性显着低于他们的nondiapausing同行。在非滞育蚊子中使用RNA干扰抑制编码胰岛素样肽-1和-5的基因，并监测卵巢成熟。在非滞育蚊子中用RNAi敲低胰岛素样肽-1导致卵巢发育停止，类似于滞育，并且这种发育停滞可以通过应用JH来逆转。敲低胰岛素样肽-5并不改变卵巢发育。这些结果与胰岛素样肽-1在信号通路中的作用是一致的，该信号通路导致从短日照长度的感知到JH产生的关闭，这是C. pipiens。
Short day-length is used to programme adult diapause in the mosquito, Culex pipiens. The downstream endocrine event that halts ovarian maturation is a shutdown in juvenile hormone (JH) production, and recent evidence suggests that the insulin signalling pathway may be a key upstream player in executing this developmental arrest. Genes encoding insulin-like peptides-1, -2 and -5 were identified in C. pipiens, and we report that transcript levels of insulin-like peptides-1 and -5 were significantly lower in diapausing females than in their nondiapausing counterparts. Genes encoding both insulin-like peptides-1 and -5 were suppressed using RNA interference in mosquitoes programmed for nondiapause, and ovarian maturation was monitored. Knocking down insulin-like peptide-1 with RNAi in nondiapausing mosquitoes resulted in a cessation of ovarian development akin to diapause, and this arrest in development could be reversed with an application of JH. Knocking down insulin-like peptide-5 did not alter ovarian development. These results are consistent with a role for insulin-like peptide-1 in the signalling pathway leading from the perception of short day-lengths to the shut-down in JH production that characterizes adult diapause in C. pipiens.
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DOI：
10.1073/pnas.0507958102
发表时间：
2005-11-01
影响因子：
11.1
作者：
Robich, RM;Denlinger, DL
通讯作者：
Denlinger, DL
影响因子：
3.8
作者：
Riehle, MA;Brown, MR
通讯作者：
Brown, MR
DOI：
10.1073/pnas.0800478105
发表时间：
2008-04-15
影响因子：
11.1
作者：
Brown, Mark R.;Clark, Kevin D.;Strand, Michael R.
通讯作者：
Strand, Michael R.
影响因子：
3
作者：
Riehle, Michael A.;Fan, Yongliang;Brown, Mark R.
通讯作者：
Brown, Mark R.
影响因子：
64.5
作者：
Renn, SCP;Park, JH;Taghert, PH
通讯作者：
Taghert, PH