Light-driven microrobotics and their applications for single-cell manipulation
Light-driven microrobotics and their applications for single-cell manipulation
复制标题
光驱动微型机器人及其在单细胞操作中的应用
DOI：
10.1117/12.2570444
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发表时间：
2020
期刊：
影响因子：
3.5
通讯作者：
A. R. Wheeler
中科院分区：
文献类型：
--
作者：
Shuailong Zhang;A. R. Wheeler
由于需要控制单个细胞和其他微米级颗粒的位置，微型机器人技术正在迅速普及。有许多基于光镊的微型机器人的例子，在这里，我们介绍的第一个微机器人系统，依赖于光电镊子（OET）的相关技术。这里描述的光电微型机器人制造简单，可以编程进行复杂的多轴操作。一个特别有用的程序是“装载”、“运输”和“输送”的串联组合，它可以应用于操纵各种微米尺寸的有效载荷。重要的是，以这种方式编程的微型机器人对脆弱的哺乳动物细胞比传统的OET技术温和得多。本文描述的微型机器人系统被证明可用于单细胞分离，克隆扩增和RNA测序，这些应用在后CRISPR生命科学研究领域变得越来越重要。我们建议，OET微型机器人系统，它可以使用显微镜和消费级光学投影仪实现，将是有用的广泛的应用在生命科学和超越。
Micro-robotics is exploding in popularity, driven by the need to control the position of individual cells and other micronsized particles. There are many examples of optical tweezer-based micro-robots; here we introduce the first microrobotic system that relies on the related technology of optoelectronic tweezers (OET). The optoelectronic micro-robots described here are straightforward to manufacture and can be programmed to carry out sophisticated, multi-axis operations. One particularly useful program is a serial combination of “load,” “transport,” and “deliver,” which can be applied to manipulate a wide range of micron-dimension payloads. Importantly, micro-robots programmed in this manner are much gentler on fragile mammalian cells than conventional OET techniques. The micro-robotic system described here was demonstrated to be useful for single-cell isolation, clonal expansion and RNA sequencing, applications that are becoming increasingly important in the post-CRISPR life-science research landscape. We propose that the OET micro-robotic system, which can be implemented using a microscope and consumer-grade optical projector, will be useful for a wide range of applications in the life sciences and beyond.
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影响因子：
35
作者：
Jamshidi, Arash;Pauzauskie, Peter J.;Wu, Ming C.
通讯作者：
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影响因子：
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影响因子：
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通讯作者：
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影响因子：
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作者：
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